The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers

Mohammad Sanami, India Sweeney, Zvi Shtein, Sigal Meirovich, Anna Sorushanova, Anne Maria Mullen, Mohsen Miraftab, Oded Shoseyov, Colm O'Dowd, Abhay Pandit, Dimitrios I. Zeugolis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Various chemical, natural, or synthetic in origin, crosslinking methods have been proposed over the years to stabilise collagen fibers. However, an optimal method has yet to be identified. Herein, we ventured to assess the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate, as opposed to glutaraldehyde (GTA), genipin and carbodiimide, on the structural, physical and biological properties of collagen fibers. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate induced an intermedium surface smoothness, denaturation temperature and swelling. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers had significantly higher stress at break values than the carbodiimide fibers, but significantly lower than the GTA and genipin fibers. With respect to strain at break, no significant difference was observed among the crosslinking treatments. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers exhibited significantly higher cell metabolic activity and DNA concentration that all other crosslinking treatments, promoted consistently cellular elongation along the longitudinal fiber axis and by day 7 they were completely covered by cells. Collectively, this work clearly demonstrates the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate as collagen crosslinker.

Original languageAmerican English
Pages (from-to)914-922
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume104
Issue number5
DOIs
StatePublished - 1 Jul 2016

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.

Keywords

  • collagen crosslinking
  • collagen fibers
  • cytocompatibility
  • mechanical properties
  • starPEG

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