The influence of the peptide chain length on the activity of peptidyl-tRNA hydrolase from E. coli

Joseph Shiloach*, Shabtai Bauer, Nathan De Groot, Yehuda Lapidot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The dependence of the Vmax and Km on the length of the peptide moiety in the peptidyl-tRNA series (Gly)n-Val-tRNA, was measured in the system peptidyl-tRNA hydrolase-peptidyl-tRNA. It was found that the Km value decreases from 7.2 × 10-7 M for Gly-Val-tRNA to 4.6 × 10-7 M for (Gly)2-Val-tRNA and to 1.7 × 10-7 M for (Gly)3-Val-tRNA; further increase of the peptide chain is not followed by decrease of the Km. The Vmax values are 5.7 pmole/min/EU for Gly-Val-tRNA and 42 pmole/min/EU for (Gly)3-Val-tRNA. The enzyme activity is inhibited competitively by uncharged tRNA with a KI value of about 10-6 M. The significance of these results described in this paper, in relation to the fact that peptides and peptide esters do not inhibit the enzyme activity, and in relation to the proposed physiological role of the enzyme, is discussed.

Original languageEnglish
Pages (from-to)1941-1950
Number of pages10
JournalNucleic Acids Research
Volume2
Issue number10
DOIs
StatePublished - Oct 1975

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