TY - JOUR
T1 - The influence of the peptide chain length on the activity of peptidyl-tRNA hydrolase from E. coli
AU - Shiloach, Joseph
AU - Bauer, Shabtai
AU - De Groot, Nathan
AU - Lapidot, Yehuda
PY - 1975/10
Y1 - 1975/10
N2 - The dependence of the Vmax and Km on the length of the peptide moiety in the peptidyl-tRNA series (Gly)n-Val-tRNA, was measured in the system peptidyl-tRNA hydrolase-peptidyl-tRNA. It was found that the Km value decreases from 7.2 × 10-7 M for Gly-Val-tRNA to 4.6 × 10-7 M for (Gly)2-Val-tRNA and to 1.7 × 10-7 M for (Gly)3-Val-tRNA; further increase of the peptide chain is not followed by decrease of the Km. The Vmax values are 5.7 pmole/min/EU for Gly-Val-tRNA and 42 pmole/min/EU for (Gly)3-Val-tRNA. The enzyme activity is inhibited competitively by uncharged tRNA with a KI value of about 10-6 M. The significance of these results described in this paper, in relation to the fact that peptides and peptide esters do not inhibit the enzyme activity, and in relation to the proposed physiological role of the enzyme, is discussed.
AB - The dependence of the Vmax and Km on the length of the peptide moiety in the peptidyl-tRNA series (Gly)n-Val-tRNA, was measured in the system peptidyl-tRNA hydrolase-peptidyl-tRNA. It was found that the Km value decreases from 7.2 × 10-7 M for Gly-Val-tRNA to 4.6 × 10-7 M for (Gly)2-Val-tRNA and to 1.7 × 10-7 M for (Gly)3-Val-tRNA; further increase of the peptide chain is not followed by decrease of the Km. The Vmax values are 5.7 pmole/min/EU for Gly-Val-tRNA and 42 pmole/min/EU for (Gly)3-Val-tRNA. The enzyme activity is inhibited competitively by uncharged tRNA with a KI value of about 10-6 M. The significance of these results described in this paper, in relation to the fact that peptides and peptide esters do not inhibit the enzyme activity, and in relation to the proposed physiological role of the enzyme, is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0016801804&partnerID=8YFLogxK
U2 - 10.1093/nar/2.10.1941
DO - 10.1093/nar/2.10.1941
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C2 - 1103091
AN - SCOPUS:0016801804
SN - 0305-1048
VL - 2
SP - 1941
EP - 1950
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 10
ER -