Honey bee queens show extreme fecundity, commonly laying more than a thousand eggs in a single day. It has proven challenging to study the temporal organization of egg-laying behavior because queens are typically active around the clock in the dark cavity of a densely populated nest. To contend with this challenge, we developed two novel methods allowing detailed monitoring of queen activity and egg laying. We first adapted a high-resolution, continuous, tracking system allowing to track the position of barcode-tagged queens in observation hives with colonies foraging outside. We found that the queen is active ~96% of the day with typically no diurnal rhythm. Next, we developed a new laboratory procedure to monitor egg laying at single egg resolution under different light regimes. We found that under constant darkness (DD) and temperature conditions, queens laid eggs with no circadian rhythms. Queen fecundity was severely reduced under constant light (LL). Under a 12:12 illumination regime, queen fecundity was comparable to under constant darkness, with a higher number of eggs during the light phase. These daily rhythms in egg laying continued when these queens were released to DD conditions, suggesting that egg-laying rhythms are influenced by endogenous circadian clocks. These results suggest that honey bee queens are active and lay eggs around the clock with no diurnal rhythms. Light has complex influences on these behaviors, but more studies are needed to determine whether these effects reflect the influence of light directly on the queen or indirectly by affecting workers that interact with the queen.
Bibliographical noteFunding Information:
We thank Ran Nathan and Yoav Bartan for lending the cameras used in this study. This project was supported by a grant from the Defense Advanced Research Projects Agency (grant number: HR0011-16-2-0019 to Gene E. Robinson and Huimin Zhao), a grant for the Israel Science Foundation (grant number: 1274/15 to Guy Bloch), and the Yitzhak Shamir postdoctoral fellowship of the Israeli Ministry of Science, Technology and Space (to Hagai Y. Shpigler).
© 2022 The Author(s).
- circadian rhythm
- egg laying
- honey bee
- illumination regime