The internal aqueous volume of small unilamellar vesicles changes at the phase transition temperature of the phospholipid

James L. Slater; Dov Lichtenberg; Thomas E. Thompson

doi:10.1016/0005-2736(83)90083-4

The internal aqueous volume of small unilamellar vesicles changes at the phase transition temperature of the phospholipid

James L. Slater^*
, Dov Lichtenberg
, Thomas E. Thompson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Changes in the fluorescence of partially self-quenched 5(6)-carboxyfluorescein trapped within the internal aqueous compartment of small unilamellar dipalmitoylphosphatidylcholine vesicles indicate that the trapped volume of these vesicles decreases when the phospholipid undergoes the liquid crystalline to gel state transition. This volume change is completely reversible and is not caused by vesicle-vesicle fusion. Furthermore, this decrease in volume of the internal aqueous compartment may be attributed to a change in vesicle shape upon undergoing the phase transition.

Original language	English
Pages (from-to)	125-128
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	734
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(83)90083-4
State	Published - 21 Sep 1983
Externally published	Yes

Keywords

Fluorescence
Phase transition
Phospholipid vesicle
Small unilamellar vesicle
Trapped volume
Vesicle shape

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10.1016/0005-2736(83)90083-4

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title = "The internal aqueous volume of small unilamellar vesicles changes at the phase transition temperature of the phospholipid",

abstract = "Changes in the fluorescence of partially self-quenched 5(6)-carboxyfluorescein trapped within the internal aqueous compartment of small unilamellar dipalmitoylphosphatidylcholine vesicles indicate that the trapped volume of these vesicles decreases when the phospholipid undergoes the liquid crystalline to gel state transition. This volume change is completely reversible and is not caused by vesicle-vesicle fusion. Furthermore, this decrease in volume of the internal aqueous compartment may be attributed to a change in vesicle shape upon undergoing the phase transition.",

keywords = "Fluorescence, Phase transition, Phospholipid vesicle, Small unilamellar vesicle, Trapped volume, Vesicle shape",

author = "Slater, \{James L.\} and Dov Lichtenberg and Thompson, \{Thomas E.\}",

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T1 - The internal aqueous volume of small unilamellar vesicles changes at the phase transition temperature of the phospholipid

AU - Slater, James L.

AU - Lichtenberg, Dov

AU - Thompson, Thomas E.

PY - 1983/9/21

Y1 - 1983/9/21

N2 - Changes in the fluorescence of partially self-quenched 5(6)-carboxyfluorescein trapped within the internal aqueous compartment of small unilamellar dipalmitoylphosphatidylcholine vesicles indicate that the trapped volume of these vesicles decreases when the phospholipid undergoes the liquid crystalline to gel state transition. This volume change is completely reversible and is not caused by vesicle-vesicle fusion. Furthermore, this decrease in volume of the internal aqueous compartment may be attributed to a change in vesicle shape upon undergoing the phase transition.

AB - Changes in the fluorescence of partially self-quenched 5(6)-carboxyfluorescein trapped within the internal aqueous compartment of small unilamellar dipalmitoylphosphatidylcholine vesicles indicate that the trapped volume of these vesicles decreases when the phospholipid undergoes the liquid crystalline to gel state transition. This volume change is completely reversible and is not caused by vesicle-vesicle fusion. Furthermore, this decrease in volume of the internal aqueous compartment may be attributed to a change in vesicle shape upon undergoing the phase transition.

KW - Fluorescence

KW - Phase transition

KW - Phospholipid vesicle

KW - Small unilamellar vesicle

KW - Trapped volume

KW - Vesicle shape

UR - https://www.scopus.com/pages/publications/4243995630

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SP - 125

EP - 128

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 1

ER -

The internal aqueous volume of small unilamellar vesicles changes at the phase transition temperature of the phospholipid

Abstract

Keywords

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