The kinetics of glucose uptake by isolated fat cells and the influence of insulin

Christine P.J. Caygill; Wilfred D. Stein

doi:10.1007/BF01564591

The kinetics of glucose uptake by isolated fat cells and the influence of insulin

Christine P.J. Caygill^*, Wilfred D. Stein

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

From measurements of glucose uptake by rat adipocytes, values of K (half-saturation constant) and V (maximal uptake at saturating glucose concentrations) are calculated. Insulin increases glucose uptake. Mannose and 3-O-methylglucose inhibit uptake. Different cell preparations give different values for K, V and insulin sensitivity. The possible effect on these differing parameters of adipocyte triglyceride content is considered. However the non-linearity of some double-reciprocal plots and certain correlations between the various kinetic parameters lead to the suggestion that rat adipocytes may have two systems for glucose uptake.

Original language	English
Pages (from-to)	900-923
Number of pages	24
Journal	Acta Diabetologica Latina
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/BF01564591
State	Published - Dec 1972
Externally published	Yes

Keywords

Fat cells
Glucose uptake
Insulin
Kinetic constants
Rat epididymal tissue
Second order kinetics

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10.1007/BF01564591

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AB - From measurements of glucose uptake by rat adipocytes, values of K (half-saturation constant) and V (maximal uptake at saturating glucose concentrations) are calculated. Insulin increases glucose uptake. Mannose and 3-O-methylglucose inhibit uptake. Different cell preparations give different values for K, V and insulin sensitivity. The possible effect on these differing parameters of adipocyte triglyceride content is considered. However the non-linearity of some double-reciprocal plots and certain correlations between the various kinetic parameters lead to the suggestion that rat adipocytes may have two systems for glucose uptake.

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KW - Glucose uptake

KW - Insulin

KW - Kinetic constants

KW - Rat epididymal tissue

KW - Second order kinetics

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The kinetics of glucose uptake by isolated fat cells and the influence of insulin

Abstract

Keywords

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