The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants

J. N. Nocillado; J. Biran; Y. Y. Lee; B. Levavi-Sivan; A. S. Mechaly; Y. Zohar; A. Elizur

doi:10.1016/j.mce.2012.06.024

The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants

J. N. Nocillado, J. Biran, Y. Y. Lee, B. Levavi-Sivan, A. S. Mechaly, Y. Zohar, A. Elizur^*

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The kisspeptin system plays an essential role in reproductive function in vertebrates, particularly in the onset of puberty. We investigated the kisspeptin system in two Perciform teleosts, the Southern Bluefin Tuna (SBT; Thunnus maccoyii), and the Yellowtail Kingfish (YTK; Seriola lalandi), by characterising their kisspeptin 2 receptor (Kiss2r) genes. In addition to the full length Kiss2r cDNA sequences, we have isolated from SBT and YTK a transcript variant that retained an intron. We have further obtained three ytk. Kiss2r transcript variants that contained deletions. In vitro functional analysis of the full length SBT and YTK Kiss2r showed higher response to Kiss2-10 than to Kiss1-10, with stronger transduction via PKC than PKA. The full length ytk. Kiss2r and two deletion variants were differentially expressed in the brain of male, but not in female, juvenile YTK treated with increasing doses of Kiss2-10 peptide. In the gonads, the expression level of the ytk. Kiss2r transcripts did not vary significantly either in the male or female fish. This is the first time that transcript variants of the Kiss2r gene that contain deletions and show responsiveness to treatments with kisspeptin have been reported in any teleost.

Original language	English
Pages (from-to)	211-220
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	362
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2012.06.024
State	Published - 15 Oct 2012

Bibliographical note

Funding Information:
This project was generously supported by a grant from the Australian Seafood CRC (2008/745) to AE and JN, and by Fisheries Research Development Centre and the University of the Sunshine Coast. We acknowledge CleanSeas Tuna Pty Ltd., South Australia, for kindly providing tissue samples and experimental fish. We thank Mr. Rafael Rodríguez (Instituto de Acuicultura de Torre de la Sal) for contributing to the molecular work. We are grateful to the staff of Lincoln Marine Science Centre, especially Dr. Trent D’Antignana, Dr. Erin Bubner and Mr. Claudio Giordano for their assistance. J. Biran was supported by an Australia-Israel Scientific Exchange Foundation Fellowship. A.S. Mechaly was supported by a predoctoral scholarship from the Spanish Ministry of Education and Science.

Keywords

Kiss2r isoforms
Kisspeptin
Puberty

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10.1016/j.mce.2012.06.024

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Nocillado, J. N., Biran, J., Lee, Y. Y., Levavi-Sivan, B., Mechaly, A. S., Zohar, Y., & Elizur, A. (2012). The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants. Molecular and Cellular Endocrinology, 362(1-2), 211-220. https://doi.org/10.1016/j.mce.2012.06.024

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abstract = "The kisspeptin system plays an essential role in reproductive function in vertebrates, particularly in the onset of puberty. We investigated the kisspeptin system in two Perciform teleosts, the Southern Bluefin Tuna (SBT; Thunnus maccoyii), and the Yellowtail Kingfish (YTK; Seriola lalandi), by characterising their kisspeptin 2 receptor (Kiss2r) genes. In addition to the full length Kiss2r cDNA sequences, we have isolated from SBT and YTK a transcript variant that retained an intron. We have further obtained three ytk. Kiss2r transcript variants that contained deletions. In vitro functional analysis of the full length SBT and YTK Kiss2r showed higher response to Kiss2-10 than to Kiss1-10, with stronger transduction via PKC than PKA. The full length ytk. Kiss2r and two deletion variants were differentially expressed in the brain of male, but not in female, juvenile YTK treated with increasing doses of Kiss2-10 peptide. In the gonads, the expression level of the ytk. Kiss2r transcripts did not vary significantly either in the male or female fish. This is the first time that transcript variants of the Kiss2r gene that contain deletions and show responsiveness to treatments with kisspeptin have been reported in any teleost.",

keywords = "Kiss2r isoforms, Kisspeptin, Puberty",

author = "Nocillado, {J. N.} and J. Biran and Lee, {Y. Y.} and B. Levavi-Sivan and Mechaly, {A. S.} and Y. Zohar and A. Elizur",

note = "Funding Information: This project was generously supported by a grant from the Australian Seafood CRC (2008/745) to AE and JN, and by Fisheries Research Development Centre and the University of the Sunshine Coast. We acknowledge CleanSeas Tuna Pty Ltd., South Australia, for kindly providing tissue samples and experimental fish. We thank Mr. Rafael Rodr{\'i}guez (Instituto de Acuicultura de Torre de la Sal) for contributing to the molecular work. We are grateful to the staff of Lincoln Marine Science Centre, especially Dr. Trent D{\textquoteright}Antignana, Dr. Erin Bubner and Mr. Claudio Giordano for their assistance. J. Biran was supported by an Australia-Israel Scientific Exchange Foundation Fellowship. A.S. Mechaly was supported by a predoctoral scholarship from the Spanish Ministry of Education and Science. ",

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Nocillado, JN, Biran, J, Lee, YY, Levavi-Sivan, B, Mechaly, AS, Zohar, Y & Elizur, A 2012, 'The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants', Molecular and Cellular Endocrinology, vol. 362, no. 1-2, pp. 211-220. https://doi.org/10.1016/j.mce.2012.06.024

The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants. / Nocillado, J. N.; Biran, J.; Lee, Y. Y. et al.
In: Molecular and Cellular Endocrinology, Vol. 362, No. 1-2, 15.10.2012, p. 211-220.

Research output: Contribution to journal › Article › peer-review

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AU - Elizur, A.

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The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi - Functional analysis and isolation of transcript variants

Abstract

Bibliographical note

Keywords

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