The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Evelyne Zeira; Rinat Abramovitch; Karen Meir; Sharona Even Ram; Yaniv Gil; Baruch Bulvik; Zohar Bromberg; Or Levkovitch; Nathalie Nahmansson; Revital Adar; Benjamin Reubinoff; Eithan Galun; Michal Gropp

doi:10.18632/oncotarget.5787

The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Evelyne Zeira, Rinat Abramovitch, Karen Meir, Sharona Even Ram, Yaniv Gil, Baruch Bulvik, Zohar Bromberg, Or Levkovitch, Nathalie Nahmansson, Revital Adar, Benjamin Reubinoff, Eithan Galun, Michal Gropp^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

Original language	American English
Pages (from-to)	34691-34703
Number of pages	13
Journal	Oncotarget
Volume	6
Issue number	33
DOIs	https://doi.org/10.18632/oncotarget.5787
State	Published - 2015
Externally published	Yes

Keywords

H19lncRNA
HEC cells
Oncogenesis
Pluripotency

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.5787

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Zeira, E., Abramovitch, R., Meir, K., Ram, S. E., Gil, Y., Bulvik, B., Bromberg, Z., Levkovitch, O., Nahmansson, N., Adar, R., Reubinoff, B., Galun, E., & Gropp, M. (2015). The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells. Oncotarget, 6(33), 34691-34703. https://doi.org/10.18632/oncotarget.5787

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title = "The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells",

abstract = "The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.",

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author = "Evelyne Zeira and Rinat Abramovitch and Karen Meir and Ram, {Sharona Even} and Yaniv Gil and Baruch Bulvik and Zohar Bromberg and Or Levkovitch and Nathalie Nahmansson and Revital Adar and Benjamin Reubinoff and Eithan Galun and Michal Gropp",

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T1 - The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

AU - Zeira, Evelyne

AU - Abramovitch, Rinat

AU - Meir, Karen

AU - Ram, Sharona Even

AU - Gil, Yaniv

AU - Bulvik, Baruch

AU - Bromberg, Zohar

AU - Levkovitch, Or

AU - Nahmansson, Nathalie

AU - Adar, Revital

AU - Reubinoff, Benjamin

AU - Galun, Eithan

AU - Gropp, Michal

PY - 2015

Y1 - 2015

N2 - The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

AB - The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

KW - H19lncRNA

KW - HEC cells

KW - Oncogenesis

KW - Pluripotency

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The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Abstract

Keywords

UN SDGs

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