TY - JOUR
T1 - The light response of drosophila photoreceptors is accompanied by an increase in cellular calcium
T2 - Effects of specific mutations
AU - Peretz, A.
AU - Suss-Toby, E.
AU - Rom-Glas, A.
AU - Arnon, A.
AU - Payne, R.
AU - Minke, B.
PY - 1994/6
Y1 - 1994/6
N2 - Photoreceptors of dissociated Drosophila retinae were loaded with the fluorescent Ca2+ indicators, fluo-3 and Calcium Green-5N. In fluo-3-loaded, wild-type photoreceptors, a rapid increase in fluorescence (Ca2+ signal) accompanied the light-evoked inward current. Removal of extracellular Ca2+ greatly reduced the Ca2+ signal, indicating Ca2+ influx as its major cause. In Calcium Green-5N-loaded trp mutants, which lack a large fraction of the Ca2+ permeability underlying the light-evoked inward current, the Ca2+ signal was smaller relative to wild-type photoreceptors. Fluo-3-loaded norpA mutant photoreceptors, which lack a light-activated phospholipase C, generated no light-evoked inward current and no Ca2+ signal. The phosphoinositide pathway therefore appears necessary for both excitation and changes in cytosolic free Ca2+ concentration.
AB - Photoreceptors of dissociated Drosophila retinae were loaded with the fluorescent Ca2+ indicators, fluo-3 and Calcium Green-5N. In fluo-3-loaded, wild-type photoreceptors, a rapid increase in fluorescence (Ca2+ signal) accompanied the light-evoked inward current. Removal of extracellular Ca2+ greatly reduced the Ca2+ signal, indicating Ca2+ influx as its major cause. In Calcium Green-5N-loaded trp mutants, which lack a large fraction of the Ca2+ permeability underlying the light-evoked inward current, the Ca2+ signal was smaller relative to wild-type photoreceptors. Fluo-3-loaded norpA mutant photoreceptors, which lack a light-activated phospholipase C, generated no light-evoked inward current and no Ca2+ signal. The phosphoinositide pathway therefore appears necessary for both excitation and changes in cytosolic free Ca2+ concentration.
UR - http://www.scopus.com/inward/record.url?scp=0028284467&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(94)90442-1
DO - 10.1016/0896-6273(94)90442-1
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C2 - 8011336
AN - SCOPUS:0028284467
SN - 0896-6273
VL - 12
SP - 1257
EP - 1267
JO - Neuron
JF - Neuron
IS - 6
ER -