The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa

J. Hans Sietsma; Adi Beth Din; Vered Ziv; Klaas A. Sjollema; Oded Yarden

doi:10.1099/13500872-142-7-1591

The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa

J. Hans Sietsma, Adi Beth Din, Vered Ziv, Klaas A. Sjollema, Oded Yarden^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Polyclonal anti-chitin synthase antibodies raised against the Saccharomyces cerevisiae CHS2 gene product were used to identify and localize chitin synthase in the filamentous ascomycete Neurospora crassa. A single band of approximately 110 kDa was observed in Western blots of total protein extracts of N. crassa, probed with these antibodies. However, several additional bands were labelled when membrane fraction proteins (microsomes) were probed. Histo-immunochemical localization of chitin synthase confirmed that the polypeptide is compartmentalized in membranous vesicles (chitosomes), which are abundant in the vicinity of the hyphal tip. TEM analysis did not reveal chitin synthase in the plasma membrane. However, dense labelling of membrane-associated chitin synthase was observed by light-microscopic analysis of N. crassa protoplasts and at young hyphal tips.

Original language	English
Pages (from-to)	1591-1596
Number of pages	6
Journal	Microbiology
Volume	142
Issue number	7
DOIs	https://doi.org/10.1099/13500872-142-7-1591
State	Published - Jul 1996

Keywords

Cell wall biosynthesis
Hyphal growth
Protoplast

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title = "The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa",

abstract = "Polyclonal anti-chitin synthase antibodies raised against the Saccharomyces cerevisiae CHS2 gene product were used to identify and localize chitin synthase in the filamentous ascomycete Neurospora crassa. A single band of approximately 110 kDa was observed in Western blots of total protein extracts of N. crassa, probed with these antibodies. However, several additional bands were labelled when membrane fraction proteins (microsomes) were probed. Histo-immunochemical localization of chitin synthase confirmed that the polypeptide is compartmentalized in membranous vesicles (chitosomes), which are abundant in the vicinity of the hyphal tip. TEM analysis did not reveal chitin synthase in the plasma membrane. However, dense labelling of membrane-associated chitin synthase was observed by light-microscopic analysis of N. crassa protoplasts and at young hyphal tips.",

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AU - Yarden, Oded

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KW - Protoplast

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The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa

Abstract

Keywords

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