The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa

J. Hans Sietsma, Adi Beth Din, Vered Ziv, Klaas A. Sjollema, Oded Yarden*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Polyclonal anti-chitin synthase antibodies raised against the Saccharomyces cerevisiae CHS2 gene product were used to identify and localize chitin synthase in the filamentous ascomycete Neurospora crassa. A single band of approximately 110 kDa was observed in Western blots of total protein extracts of N. crassa, probed with these antibodies. However, several additional bands were labelled when membrane fraction proteins (microsomes) were probed. Histo-immunochemical localization of chitin synthase confirmed that the polypeptide is compartmentalized in membranous vesicles (chitosomes), which are abundant in the vicinity of the hyphal tip. TEM analysis did not reveal chitin synthase in the plasma membrane. However, dense labelling of membrane-associated chitin synthase was observed by light-microscopic analysis of N. crassa protoplasts and at young hyphal tips.

Original languageAmerican English
Pages (from-to)1591-1596
Number of pages6
JournalMicrobiology
Volume142
Issue number7
DOIs
StatePublished - Jul 1996

Keywords

  • Cell wall biosynthesis
  • Hyphal growth
  • Protoplast

Fingerprint

Dive into the research topics of 'The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa'. Together they form a unique fingerprint.

Cite this