The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere

O. A. Podosokorskaya; A. V. Teplyuk; K. S. Zayulina; D. S. Kopitsyn; I. N. Dominova; A. G. Elcheninov; S. V. Toshchakov; I. V. Kublanov

doi:10.1134/S0026261719050126

The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere

O. A. Podosokorskaya^*, A. V. Teplyuk, K. S. Zayulina, D. S. Kopitsyn, I. N. Dominova, A. G. Elcheninov, S. V. Toshchakov, I. V. Kublanov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Abstract: A novel moderately thermophilic, organotrophic bacterium, strain Par-f-2, was isolated from a sample of hot water emerging from a 2775 m-deep well in Western Siberia, Russia. Cells of the novel isolate were non-sporulating Gram-negative motile rods, often forming groups and aggregates. Strain Par-f-2 grew at 25–60°C, pH 6.0–9.5, and at NaCl concentration from 5 to 7 g L^–1. The isolate is an aerobe capable of utilizing mono- and polysaccharides, yeast extract, and some organic acids and aminoacids as the carbon and energy sources. Microaerobic growth was observed. Elemental sulfur, sulfate, nitrate, fumarate, and arsenate were not reduced. The major fatty acids were C_16:0 (69.7%) and C_16:1ω7 (19.3%). The G+C content of the DNA was 63.5 mol%. 16S rRNA gene sequence-based phylogenetic analysis showed Thauera hydrothermalis (Betaproteobacteria) being its closest relative with 99.8% of 16S rRNA gene sequences identity. Strain Par-f-2 was deposited in the DSMZ and VKM (=DSM 26751=VKM V-2822).

Original language	English
Pages (from-to)	556-562
Number of pages	7
Journal	Microbiology (Russian Federation)
Volume	88
Issue number	5
DOIs	https://doi.org/10.1134/S0026261719050126
State	Published - 1 Sep 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019, Pleiades Publishing, Ltd.

Keywords

Thauera hydrothermalis
aerobe
hydrolytic bacteria
subsurface biosphere
thermophile

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10.1134/S0026261719050126

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Podosokorskaya, O. A., Teplyuk, A. V., Zayulina, K. S., Kopitsyn, D. S., Dominova, I. N., Elcheninov, A. G., Toshchakov, S. V., & Kublanov, I. V. (2019). The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere. Microbiology (Russian Federation), 88(5), 556-562. https://doi.org/10.1134/S0026261719050126

@article{10aea8f2642641d593af5e23975ec8ea,

title = "The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere",

abstract = "Abstract: A novel moderately thermophilic, organotrophic bacterium, strain Par-f-2, was isolated from a sample of hot water emerging from a 2775 m-deep well in Western Siberia, Russia. Cells of the novel isolate were non-sporulating Gram-negative motile rods, often forming groups and aggregates. Strain Par-f-2 grew at 25–60°C, pH 6.0–9.5, and at NaCl concentration from 5 to 7 g L–1. The isolate is an aerobe capable of utilizing mono- and polysaccharides, yeast extract, and some organic acids and aminoacids as the carbon and energy sources. Microaerobic growth was observed. Elemental sulfur, sulfate, nitrate, fumarate, and arsenate were not reduced. The major fatty acids were C16:0 (69.7%) and C16:1ω7 (19.3%). The G+C content of the DNA was 63.5 mol%. 16S rRNA gene sequence-based phylogenetic analysis showed Thauera hydrothermalis (Betaproteobacteria) being its closest relative with 99.8% of 16S rRNA gene sequences identity. Strain Par-f-2 was deposited in the DSMZ and VKM (=DSM 26751=VKM V-2822).",

keywords = "Thauera hydrothermalis, aerobe, hydrolytic bacteria, subsurface biosphere, thermophile",

author = "Podosokorskaya, {O. A.} and Teplyuk, {A. V.} and Zayulina, {K. S.} and Kopitsyn, {D. S.} and Dominova, {I. N.} and Elcheninov, {A. G.} and Toshchakov, {S. V.} and Kublanov, {I. V.}",

note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Ltd.",

year = "2019",

month = sep,

day = "1",

doi = "10.1134/S0026261719050126",

language = "אנגלית",

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Podosokorskaya, OA, Teplyuk, AV, Zayulina, KS, Kopitsyn, DS, Dominova, IN, Elcheninov, AG, Toshchakov, SV & Kublanov, IV 2019, 'The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere', Microbiology (Russian Federation), vol. 88, no. 5, pp. 556-562. https://doi.org/10.1134/S0026261719050126

TY - JOUR

T1 - The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere

AU - Podosokorskaya, O. A.

AU - Teplyuk, A. V.

AU - Zayulina, K. S.

AU - Kopitsyn, D. S.

AU - Dominova, I. N.

AU - Elcheninov, A. G.

AU - Toshchakov, S. V.

AU - Kublanov, I. V.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Abstract: A novel moderately thermophilic, organotrophic bacterium, strain Par-f-2, was isolated from a sample of hot water emerging from a 2775 m-deep well in Western Siberia, Russia. Cells of the novel isolate were non-sporulating Gram-negative motile rods, often forming groups and aggregates. Strain Par-f-2 grew at 25–60°C, pH 6.0–9.5, and at NaCl concentration from 5 to 7 g L–1. The isolate is an aerobe capable of utilizing mono- and polysaccharides, yeast extract, and some organic acids and aminoacids as the carbon and energy sources. Microaerobic growth was observed. Elemental sulfur, sulfate, nitrate, fumarate, and arsenate were not reduced. The major fatty acids were C16:0 (69.7%) and C16:1ω7 (19.3%). The G+C content of the DNA was 63.5 mol%. 16S rRNA gene sequence-based phylogenetic analysis showed Thauera hydrothermalis (Betaproteobacteria) being its closest relative with 99.8% of 16S rRNA gene sequences identity. Strain Par-f-2 was deposited in the DSMZ and VKM (=DSM 26751=VKM V-2822).

AB - Abstract: A novel moderately thermophilic, organotrophic bacterium, strain Par-f-2, was isolated from a sample of hot water emerging from a 2775 m-deep well in Western Siberia, Russia. Cells of the novel isolate were non-sporulating Gram-negative motile rods, often forming groups and aggregates. Strain Par-f-2 grew at 25–60°C, pH 6.0–9.5, and at NaCl concentration from 5 to 7 g L–1. The isolate is an aerobe capable of utilizing mono- and polysaccharides, yeast extract, and some organic acids and aminoacids as the carbon and energy sources. Microaerobic growth was observed. Elemental sulfur, sulfate, nitrate, fumarate, and arsenate were not reduced. The major fatty acids were C16:0 (69.7%) and C16:1ω7 (19.3%). The G+C content of the DNA was 63.5 mol%. 16S rRNA gene sequence-based phylogenetic analysis showed Thauera hydrothermalis (Betaproteobacteria) being its closest relative with 99.8% of 16S rRNA gene sequences identity. Strain Par-f-2 was deposited in the DSMZ and VKM (=DSM 26751=VKM V-2822).

KW - Thauera hydrothermalis

KW - aerobe

KW - hydrolytic bacteria

KW - subsurface biosphere

KW - thermophile

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DO - 10.1134/S0026261719050126

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AN - SCOPUS:85073872952

SN - 0026-2617

VL - 88

SP - 556

EP - 562

JO - Microbiology (Russian Federation)

JF - Microbiology (Russian Federation)

IS - 5

ER -

The Metabolism of Thermophilic Hydrolytic Bacterium Thauera hydrothermalis Strain par-f-2 Isolated from the West Siberian Subsurface Biosphere

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Keywords

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