TY - JOUR
T1 - The molecular basis for sonographic cervical shortening at term
T2 - Identification of differentially expressed genes and the epithelial-mesenchymal transition as a function of cervical length
AU - Hassan, Sonia S.
AU - Romero, Roberto
AU - Tarca, Adi L.
AU - Nhan-Chang, Chia Ling
AU - Mittal, Pooja
AU - Vaisbuch, Edi
AU - Gonzalez, Juan M.
AU - Chaiworapongsa, Tinnakorn
AU - Ali-Fehmi, Rouba
AU - Dong, Zhong
AU - Than, Nandor Gabor
AU - Kim, Chong Jai
PY - 2010/11
Y1 - 2010/11
N2 - Objective The purpose of this study was to determine whether cervical shortening of a ripe cervix at term is associated with changes in the cervical transcriptome. Study Design Sonographically measured cervical lengths and biopsy specimens were obtained from 19 women at term who were not in labor with a ripe cervix. Affymetrix HG-U133 Plus 2.0 arrays (Affymetrix Inc, Santa Clara, CA) were used. Gene expression was analyzed as a function of cervical length. Gene Ontology, pathway analyses, quantitative real-time reverse transcriptionpolymerase chain reaction, and immunohistochemistry were performed. Results Cervical length shortening was associated with differential expression of 687 genes. Fifty-four biologic processes, 22 molecular functions, and 9 pathways were enriched. Quantitative real-time reverse transcriptionpolymerase chain reaction analysis confirmed differential expression of 13 genes. Bone morphogenetic protein-7, claudin-1, integrin beta-6, and endometrial progesteroneinduced protein messenger RNA, and protein expressions were down-regulated with cervical shortening. Conclusion Sonographic cervical shortening in patients at term who are not in labor with a ripe cervix is associated with changes in the uterine cervix transcriptome. The epithelial-mesenchymal transition may participate in the mechanism of cervical shortening at term.
AB - Objective The purpose of this study was to determine whether cervical shortening of a ripe cervix at term is associated with changes in the cervical transcriptome. Study Design Sonographically measured cervical lengths and biopsy specimens were obtained from 19 women at term who were not in labor with a ripe cervix. Affymetrix HG-U133 Plus 2.0 arrays (Affymetrix Inc, Santa Clara, CA) were used. Gene expression was analyzed as a function of cervical length. Gene Ontology, pathway analyses, quantitative real-time reverse transcriptionpolymerase chain reaction, and immunohistochemistry were performed. Results Cervical length shortening was associated with differential expression of 687 genes. Fifty-four biologic processes, 22 molecular functions, and 9 pathways were enriched. Quantitative real-time reverse transcriptionpolymerase chain reaction analysis confirmed differential expression of 13 genes. Bone morphogenetic protein-7, claudin-1, integrin beta-6, and endometrial progesteroneinduced protein messenger RNA, and protein expressions were down-regulated with cervical shortening. Conclusion Sonographic cervical shortening in patients at term who are not in labor with a ripe cervix is associated with changes in the uterine cervix transcriptome. The epithelial-mesenchymal transition may participate in the mechanism of cervical shortening at term.
KW - bone morphogenetic protein 7 (BMP-7)
KW - cervical length
KW - cervical ripening
KW - claudin-1
KW - endometrial progesterone-induced protein (EPIP)
KW - epithelial-mesenchymal transition
KW - extracellular matrix
KW - high-dimensional biology
KW - preterm birth
KW - progesterone
KW - PSAT1
KW - tight junction
KW - transforming growth factor-beta (TGF-β)
KW - uterine cervix
UR - http://www.scopus.com/inward/record.url?scp=78149280268&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2010.06.076
DO - 10.1016/j.ajog.2010.06.076
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 20817141
AN - SCOPUS:78149280268
SN - 0002-9378
VL - 203
SP - 472.e1-472.e14
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 5
ER -