The murine SNF5/INI1 chromatin remodeling factor is essential for embryonic development and tumor suppression

Agnes Klochendler-Yeivin, Laurence Fiette, Jaqueline Barra, Christian Muchardt, Charles Babinet, Moshe Yaniv*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

346 Scopus citations

Abstract

The assembly of eukaryotic DNA into nucleosomes and derived higher order structures constitutes a barrier for transcription, replication and repair. A number of chromatin remodeling complexes, as well as histone acetylation, were shown to facilitate gene activation. To investigate the function of two closely related mammalian SWI/SNF complexes in vivo, we inactivated the murine SNF5/INI1 gene, a common subunit of these two complexes. Mice lacking SNF5 protein stop developing at the peri-implantation stage, showing that the SWI/SNF complex is essential for early development and viability of early embryonic cells. Furthermore, heterozygous mice develop nervous system and soft tissue sarcomas. In these tumors the wild-type allele was lost, providing further evidence that SNF5 functions as a tumor suppressor gene in certain cell types.

Original languageAmerican English
Pages (from-to)500-506
Number of pages7
JournalEMBO Reports
Volume1
Issue number6
DOIs
StatePublished - 2000
Externally publishedYes

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