The natural cytotoxicity receptor NKp46 is dispensable for IL-22-mediated innate intestinal immune defense against Citrobacter rodentium

Naoko Satoh-Takayama, Laure Dumoutier, Sarah Lesjean-Pottier, Vera S.G. Ribeiro, Ofer Mandelboim, Jean Christophe Renauld, Christian A.J. Vosshenrich, James P. Di Santo

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Natural cytotoxicity receptors (including NKp30, NKp44, and NKp46 in humans and NKp46 in mice) are type I transmembrane proteins that signal NK cell activation via ITAM-containing adapter proteins in response to stress- and pathogen-induced ligands. Although murine NKp46 expression (encoded by Ncr1) was thought to be predominantly restricted to NK cells, the identification of distinct intestinal NKp46+ cell subsets that express the transcription factor Rorc and produce IL-22 suggests a broader function for NKp46 that could involve intestinal homeostasis and immune defense. Using mice carrying a GFP-modified Ncr1 allele, we found normal numbers of gut CD3 -GFP+ cells with a similar cell surface phenotype and subset distribution in the absence of Ncr1. Splenic and intestinal CD3 -NKp46+ cell subsets showed distinct patterns of cytokine secretion (IFN-γ, IL-22) following activation via NK1.1, NKp46, IL-12 plus IL-18, or IL-23. However, IL-22 production was sharply restricted to intestinal CD3-GFP+ cells with the CD127+NK1.1 - phenotype and could be induced in an Ncr1-independent fashion. Because NKp46 ligands can trigger immune activation in the context of infectious pathogens, we assessed the response of wild-type and Ncr-1-deficient Rag2 -/- mice to the enteric pathogen Citrobacter rodentium. No differences in the survival or clinical score were observed in C. rodentium-infected Rag2-/- mice lacking Ncr1, indicating that NKp46 plays a redundant role in the differentiation of intestinal IL-22+ cells that mediate innate defense against this pathogen. Our results provide further evidence for functional heterogeneity in intestinal NKp46+ cells that contrast with splenic NK cells.

Original languageAmerican English
Pages (from-to)6579-6587
Number of pages9
JournalJournal of Immunology
Volume183
Issue number10
DOIs
StatePublished - 15 Nov 2009

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