The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation

Antonia Wallrapp, Samantha J. Riesenfeld, Patrick R. Burkett, Raja Elie E. Abdulnour, Jackson Nyman, Danielle Dionne, Matan Hofree, Michael S. Cuoco, Christopher Rodman, Daneyal Farouq, Brian J. Haas, Timothy L. Tickle, John J. Trombetta, Pankaj Baral, Christoph S.N. Klose, Tanel Mahlakõiv, David Artis, Orit Rozenblatt-Rosen, Isaac M. Chiu, Bruce D. LevyMonika S. Kowalczyk, Aviv Regev, Vijay K. Kuchroo

Research output: Contribution to journalArticlepeer-review

427 Scopus citations

Abstract

Type 2 innate lymphoid cells (ILC2s) both contribute to mucosal homeostasis and initiate pathologic inflammation in allergic asthma. However, the signals that direct ILC2s to promote homeostasis versus inflammation are unclear. To identify such molecular cues, we profiled mouse lung-resident ILCs using single-cell RNA sequencing at steady state and after in vivo stimulation with the alarmin cytokines IL-25 and IL-33. ILC2s were transcriptionally heterogeneous after activation, with subpopulations distinguished by expression of proliferative, homeostatic and effector genes. The neuropeptide receptor Nmur1 was preferentially expressed by ILC2s at steady state and after IL-25 stimulation. Neuromedin U (NMU), the ligand of NMUR1, activated ILC2s in vitro, and in vivo co-administration of NMU with IL-25 strongly amplified allergic inflammation. Loss of NMU-NMUR1 signalling reduced ILC2 frequency and effector function, and altered transcriptional programs following allergen challenge in vivo. Thus, NMUR1 signalling promotes inflammatory ILC2 responses, highlighting the importance of neuro-immune crosstalk in allergic inflammation at mucosal surfaces.

Original languageAmerican English
Pages (from-to)351-356
Number of pages6
JournalNature
Volume549
Issue number7672
DOIs
StatePublished - 21 Sep 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.

Fingerprint

Dive into the research topics of 'The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation'. Together they form a unique fingerprint.

Cite this