The Neurospora crassa chs3 gene encodes an essential class I chitin synthase

Adi Beth-Din, Oded Yarden*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

In Neurospora crassa, at least five different DNA fragments related to chitin synthases have been identified. In this study we have cloned, sequenced, mapped and characterized the chitin synthase 3 structural gene (designated chs3) of N. crassa. Based on the amino acid sequence deduced from the cloned chs3 genomic DNA fragments we have designated this gene as a class I chitin synthase. The chs3 gene is transcribed predominantly at the early stages of mycelial growth and is expressed during all stages of asexual growth. The fact that strains with nuclei harboring a disrupted chs3 gene could only be maintained as heterokaryons indicates that this gene is indispensable in N. crassa.

Original languageAmerican English
Pages (from-to)65-73
Number of pages9
JournalMycologia
Volume92
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Cell wall
  • Development
  • Growth

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