TY - JOUR
T1 - The organization of the osteocyte network mirrors the extracellular matrix orientation in bone
AU - Kerschnitzki, Michael
AU - Wagermaier, Wolfgang
AU - Roschger, Paul
AU - Seto, Jong
AU - Shahar, Ron
AU - Duda, Georg N.
AU - Mundlos, Stefan
AU - Fratzl, Peter
PY - 2011/2
Y1 - 2011/2
N2 - Bone is a dynamic tissue that is continually undergoing a process of remodeling - an effect due to the interplay between bone resorption by osteoclasts and bone formation by osteoblasts. When new bone is deposited, some of the osteoblasts are embedded in the mineralizing collagen matrix and differentiate to osteocytes, forming a dense network throughout the whole bone tissue. Here, we investigate the extent to which the organization of the osteocyte network controls the collagen matrix arrangement found in various bone tissues. Several tissue types from equine, ovine and murine bone have been examined using confocal laser scanning microscopy as well as polarized light microscopy and back-scattered electron imaging. From comparing the spatial arrangements of unorganized and organized bone, we propose that the formation of a highly oriented collagen matrix requires an alignment of osteoblasts whereby a substrate layer provides a surface such that osteoblasts can align and, collectively, build new matrix. Without such a substrate, osteoblasts act isolated and only form matrices without long range order. Hence, we conclude that osteoblasts synthesize and utilize scaffold-like primary tissue as a guide for the deposition of highly ordered and mechanically competent bone tissue by a collective action of many cells.
AB - Bone is a dynamic tissue that is continually undergoing a process of remodeling - an effect due to the interplay between bone resorption by osteoclasts and bone formation by osteoblasts. When new bone is deposited, some of the osteoblasts are embedded in the mineralizing collagen matrix and differentiate to osteocytes, forming a dense network throughout the whole bone tissue. Here, we investigate the extent to which the organization of the osteocyte network controls the collagen matrix arrangement found in various bone tissues. Several tissue types from equine, ovine and murine bone have been examined using confocal laser scanning microscopy as well as polarized light microscopy and back-scattered electron imaging. From comparing the spatial arrangements of unorganized and organized bone, we propose that the formation of a highly oriented collagen matrix requires an alignment of osteoblasts whereby a substrate layer provides a surface such that osteoblasts can align and, collectively, build new matrix. Without such a substrate, osteoblasts act isolated and only form matrices without long range order. Hence, we conclude that osteoblasts synthesize and utilize scaffold-like primary tissue as a guide for the deposition of highly ordered and mechanically competent bone tissue by a collective action of many cells.
KW - Collagen matrix arrangement
KW - Confocal laser scanning microscopy
KW - Lamellar bone
KW - Osteocyte network
KW - Woven bone
UR - http://www.scopus.com/inward/record.url?scp=78751574314&partnerID=8YFLogxK
U2 - 10.1016/j.jsb.2010.11.014
DO - 10.1016/j.jsb.2010.11.014
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 21081167
AN - SCOPUS:78751574314
SN - 1047-8477
VL - 173
SP - 303
EP - 311
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 2
ER -