The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein

Aixia Zhang, Shoshy Altuvia, Anita Tiwari, Liron Argaman, Regine Hengge-Aronis, Gisela Storz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

303 Scopus citations

Abstract

The OxyS regulatory RNA integrates the adaptive response to hydrogen peroxide with other cellular stress responses and protects against DNA damage, Among the OxyS targets is the rpoS-encoded σ(s) subunit of RNA polymerase. σ(s) is a central regulator of genes induced by osmotic stress, starvation and entry into stationary phase. We examined the mechanism whereby OxyS represses rpoS expression and found that the OxyS RNA inhibits translation of the rpoS message. This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I). Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity.

Original languageAmerican English
Pages (from-to)6061-6068
Number of pages8
JournalEMBO Journal
Volume17
Issue number20
DOIs
StatePublished - 15 Oct 1998

Keywords

  • Hfq protein
  • Oxidative stress
  • OxyS RNA
  • RNA-binding protein
  • rpoS

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