Abstract
The spatial boundaries of tissue response to wounding are unknown. Here, we show that in mammals, the ribosomal protein S6 (rpS6) is phosphorylated in response to skin injury, forming a zone of activation surrounding the region of the initial insult. This p-rpS6-zone forms within minutes after wounding and is present until healing is complete. The zone is a robust marker of healing as it encapsulates features of the healing process, including proliferation, growth, cellular senescence, and angiogenesis. A mouse model that is unable to phosphorylate rpS6 shows an initial acceleration of wound closure, but results in impaired healing, identifying p-rpS6 as a modulator but not a driver of healing. Finally, the p-rpS6-zone accurately reports on the status of dermal vasculature and the effectiveness of healing, visually dividing an otherwise homogeneous tissue into regions with distinct properties.
Original language | English |
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Pages (from-to) | 981-992.e6 |
Journal | Developmental Cell |
Volume | 58 |
Issue number | 11 |
DOIs | |
State | Published - 5 Jun 2023 |
Bibliographical note
Publisher Copyright:© 2023 The Author(s)
Keywords
- cellular senescence
- immediate response
- mTOR
- p-rpS6
- p-rpS6-zone
- regeneration
- S6
- tissue damage
- wound healing
- wound marker