The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro

O. M. Tun; A. Lipsky; T. Luzzatto Knaan; Z. Kerem; I. Yedidia

doi:10.1007/s10535-012-0254-4

The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro

O. M. Tun, A. Lipsky, T. Luzzatto Knaan, Z. Kerem, I. Yedidia^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Benzothiadiazole (BTH) is a structural analogue of salicylic acid (SA) which is widely recognized for its role in elicitation of systemic acquired resistance in a broad range of plant species. Here, BTH was applied to cell cultures of the bulbous ornamental plants Ornithogalum dubium and O. thyrsoides, showing a strong effect on rates of differentiation and morphogenesis. Morphogenic cell clusters in liquid Murashige and Skoog (MS) medium containing 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) were used for all treatments. The calluses were washed thoroughly and activated with increasing concentrations of BTH. Following the induction, calli were grown on a solid MS medium without growth regulators (MS) or on a comparable media with NAA and BAP (M-206). The calli treated with BTH displayed a dose dependent increase in formation of meristematic centres followed by enhanced shoot formation compared to controls. Microscopic analyses revealed increased differentiation to cell organelles and a strengthening of the cell wall. A stronger response to BTH was observed in MS than in M-206 medium. A similar effect on calli differentiation was obtained by three weeks darkness followed by light exposure. The dark/light positive effect on differentiation was further augmented by BTH in a synergistic fashion. It is suggested that BTH enhances the rates of morphogenesis in Ornithogalum cultures by triggering a plant regulator-like activity.

Original language	English
Pages (from-to)	41-48
Number of pages	8
Journal	Biologia Plantarum
Volume	57
Issue number	1
DOIs	https://doi.org/10.1007/s10535-012-0254-4
State	Published - Mar 2013

Bibliographical note

Funding Information:
Received 7 June 2011, accepted 28 February 2012. Abbreviations: BAP - 6-benzylaminopurine; BTH - benzothiadiazole; MS - Murashige and Skoog; MS-206 - MS medium supplemented with NAA and BAP, NAA - 1-naphthaleneacetic acid; PBS - phosphate buffered saline; SA - salicylic acid. Acknowledgements: This work was supported by the Grant Agency UNESCO/ISRAEL Co-Sponsored 'Fellowships for Post-Doctoral Studies in Science and Technology. The authors thank the Chief Scientist of the Israeli Ministry of Agriculture (grant No. 256-0717) and Syngenta for kindly providing Bion® for the research. * Corresponding author; fax: (+972) 3 9669583, e-mail: [email protected]

Keywords

1-naphthaleneacetic acid
6-benzylaminopurine
benzothiadiazole
micropropagation
morphogenesis

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10.1007/s10535-012-0254-4

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title = "The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro",

abstract = "Benzothiadiazole (BTH) is a structural analogue of salicylic acid (SA) which is widely recognized for its role in elicitation of systemic acquired resistance in a broad range of plant species. Here, BTH was applied to cell cultures of the bulbous ornamental plants Ornithogalum dubium and O. thyrsoides, showing a strong effect on rates of differentiation and morphogenesis. Morphogenic cell clusters in liquid Murashige and Skoog (MS) medium containing 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) were used for all treatments. The calluses were washed thoroughly and activated with increasing concentrations of BTH. Following the induction, calli were grown on a solid MS medium without growth regulators (MS) or on a comparable media with NAA and BAP (M-206). The calli treated with BTH displayed a dose dependent increase in formation of meristematic centres followed by enhanced shoot formation compared to controls. Microscopic analyses revealed increased differentiation to cell organelles and a strengthening of the cell wall. A stronger response to BTH was observed in MS than in M-206 medium. A similar effect on calli differentiation was obtained by three weeks darkness followed by light exposure. The dark/light positive effect on differentiation was further augmented by BTH in a synergistic fashion. It is suggested that BTH enhances the rates of morphogenesis in Ornithogalum cultures by triggering a plant regulator-like activity.",

keywords = "1-naphthaleneacetic acid, 6-benzylaminopurine, benzothiadiazole, micropropagation, morphogenesis",

author = "Tun, {O. M.} and A. Lipsky and {Luzzatto Knaan}, T. and Z. Kerem and I. Yedidia",

note = "Funding Information: Received 7 June 2011, accepted 28 February 2012. Abbreviations: BAP - 6-benzylaminopurine; BTH - benzothiadiazole; MS - Murashige and Skoog; MS-206 - MS medium supplemented with NAA and BAP, NAA - 1-naphthaleneacetic acid; PBS - phosphate buffered saline; SA - salicylic acid. Acknowledgements: This work was supported by the Grant Agency UNESCO/ISRAEL Co-Sponsored 'Fellowships for Post-Doctoral Studies in Science and Technology. The authors thank the Chief Scientist of the Israeli Ministry of Agriculture (grant No. 256-0717) and Syngenta for kindly providing Bion{\textregistered} for the research. * Corresponding author; fax: (+972) 3 9669583, e-mail: [email protected]",

year = "2013",

month = mar,

doi = "10.1007/s10535-012-0254-4",

language = "אנגלית",

volume = "57",

pages = "41--48",

journal = "Biologia Plantarum",

issn = "0006-3134",

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T1 - The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro

AU - Tun, O. M.

AU - Lipsky, A.

AU - Luzzatto Knaan, T.

AU - Kerem, Z.

AU - Yedidia, I.

N1 - Funding Information: Received 7 June 2011, accepted 28 February 2012. Abbreviations: BAP - 6-benzylaminopurine; BTH - benzothiadiazole; MS - Murashige and Skoog; MS-206 - MS medium supplemented with NAA and BAP, NAA - 1-naphthaleneacetic acid; PBS - phosphate buffered saline; SA - salicylic acid. Acknowledgements: This work was supported by the Grant Agency UNESCO/ISRAEL Co-Sponsored 'Fellowships for Post-Doctoral Studies in Science and Technology. The authors thank the Chief Scientist of the Israeli Ministry of Agriculture (grant No. 256-0717) and Syngenta for kindly providing Bion® for the research. * Corresponding author; fax: (+972) 3 9669583, e-mail: [email protected]

PY - 2013/3

Y1 - 2013/3

N2 - Benzothiadiazole (BTH) is a structural analogue of salicylic acid (SA) which is widely recognized for its role in elicitation of systemic acquired resistance in a broad range of plant species. Here, BTH was applied to cell cultures of the bulbous ornamental plants Ornithogalum dubium and O. thyrsoides, showing a strong effect on rates of differentiation and morphogenesis. Morphogenic cell clusters in liquid Murashige and Skoog (MS) medium containing 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) were used for all treatments. The calluses were washed thoroughly and activated with increasing concentrations of BTH. Following the induction, calli were grown on a solid MS medium without growth regulators (MS) or on a comparable media with NAA and BAP (M-206). The calli treated with BTH displayed a dose dependent increase in formation of meristematic centres followed by enhanced shoot formation compared to controls. Microscopic analyses revealed increased differentiation to cell organelles and a strengthening of the cell wall. A stronger response to BTH was observed in MS than in M-206 medium. A similar effect on calli differentiation was obtained by three weeks darkness followed by light exposure. The dark/light positive effect on differentiation was further augmented by BTH in a synergistic fashion. It is suggested that BTH enhances the rates of morphogenesis in Ornithogalum cultures by triggering a plant regulator-like activity.

AB - Benzothiadiazole (BTH) is a structural analogue of salicylic acid (SA) which is widely recognized for its role in elicitation of systemic acquired resistance in a broad range of plant species. Here, BTH was applied to cell cultures of the bulbous ornamental plants Ornithogalum dubium and O. thyrsoides, showing a strong effect on rates of differentiation and morphogenesis. Morphogenic cell clusters in liquid Murashige and Skoog (MS) medium containing 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) were used for all treatments. The calluses were washed thoroughly and activated with increasing concentrations of BTH. Following the induction, calli were grown on a solid MS medium without growth regulators (MS) or on a comparable media with NAA and BAP (M-206). The calli treated with BTH displayed a dose dependent increase in formation of meristematic centres followed by enhanced shoot formation compared to controls. Microscopic analyses revealed increased differentiation to cell organelles and a strengthening of the cell wall. A stronger response to BTH was observed in MS than in M-206 medium. A similar effect on calli differentiation was obtained by three weeks darkness followed by light exposure. The dark/light positive effect on differentiation was further augmented by BTH in a synergistic fashion. It is suggested that BTH enhances the rates of morphogenesis in Ornithogalum cultures by triggering a plant regulator-like activity.

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The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro

Abstract

Bibliographical note

Keywords

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