The pleiotropic effects of 33258-Hoechst on the cell cycle in Chinese hamster cells in vitro

Joseph Hirschberg; Uri Lavi; Ruth Goitein; Menashe Marcus

doi:10.1016/0014-4827(80)90042-7

The pleiotropic effects of 33258-Hoechst on the cell cycle in Chinese hamster cells in vitro

Joseph Hirschberg^*, Uri Lavi, Ruth Goitein, Menashe Marcus

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

The fluorochrome 33258-Hoechst which binds to double-stranded DNA (dsDNA) has been previously shown to inhibit in several mammalian cell cultures the condensation of chromosomes in phase G2 and early mitosis. We have now found that this drug affects the cell cycle of Chinese hamster cells grown in vitro in several other ways. In cells treated with the drug, phase G2 is prolonged, the rate of DNA replication is drastically reduced and the cells are arrested most probably at very late S phase.

Original language	English
Pages (from-to)	63-72
Number of pages	10
Journal	Experimental Cell Research
Volume	130
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(80)90042-7
State	Published - 1980

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title = "The pleiotropic effects of 33258-Hoechst on the cell cycle in Chinese hamster cells in vitro",

abstract = "The fluorochrome 33258-Hoechst which binds to double-stranded DNA (dsDNA) has been previously shown to inhibit in several mammalian cell cultures the condensation of chromosomes in phase G2 and early mitosis. We have now found that this drug affects the cell cycle of Chinese hamster cells grown in vitro in several other ways. In cells treated with the drug, phase G2 is prolonged, the rate of DNA replication is drastically reduced and the cells are arrested most probably at very late S phase.",

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AU - Goitein, Ruth

AU - Marcus, Menashe

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AB - The fluorochrome 33258-Hoechst which binds to double-stranded DNA (dsDNA) has been previously shown to inhibit in several mammalian cell cultures the condensation of chromosomes in phase G2 and early mitosis. We have now found that this drug affects the cell cycle of Chinese hamster cells grown in vitro in several other ways. In cells treated with the drug, phase G2 is prolonged, the rate of DNA replication is drastically reduced and the cells are arrested most probably at very late S phase.

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The pleiotropic effects of 33258-Hoechst on the cell cycle in Chinese hamster cells in vitro

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