The reducing antioxidant capacity of Mycoplasma fermentans

Amichai Yavlovich, Ron Kohen, Isaac Ginsburg, Shlomo Rottem*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Mycoplasma fermentans is an extracellular microorganism capable of adhering to the surface of host cells. It has been recently shown that plasminogen binding to M. fermentans in the presence of the urokinase-type plasminogen activator promotes the invasion of host cells by this organism. In this report, we show that viable mycoplasmas persist within the infected HeLa cells for prolonged periods of time despite the expectation that within host cells the organism may be exposed to oxidative stress. Using cyclic voltammetry and luminol-enhanced chemiluminescence assays, we detected a potent reducing antioxidant activity in M. fermentans. The reducing antioxidant activity was heat stable, not affected by proteolysis and was almost totally lost upon dialysis suggesting that the activity is due to a nonproteinaceus low molecular weight antioxidant. This antioxidant was partially purified by Bio-Gel column chromatography followed by high-pressure liquid chromatographic analysis. We suggest that the high reducing antioxidant capacity in M. fermentans is a principal defense mechanism playing a major role in the battle of the organism against oxidative stress within the host cells.

Original languageAmerican English
Pages (from-to)195-200
Number of pages6
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Jun 2006


  • Antioxidant activity
  • Bacterial invasion
  • Chemiluminescence
  • Cyclic voltammetry
  • HeLa cells
  • Mycoplasma fermentans
  • Reducing capacity


Dive into the research topics of 'The reducing antioxidant capacity of Mycoplasma fermentans'. Together they form a unique fingerprint.

Cite this