The role of electrons’ spin in DNA oxidative damage recognition

Qirong Zhu; Yael Kapon; Aaron M. Fleming; Suryakant Mishra; Kakali Santra; Francesco Tassinari; Sidney R. Cohen; Tapan Kumar Das; Yutao Sang; Deb K. Bhowmick; Cynthia J. Burrows; Yossi Paltiel; Ron Naaman

doi:10.1016/j.xcrp.2022.101157

The role of electrons’ spin in DNA oxidative damage recognition

Qirong Zhu, Yael Kapon, Aaron M. Fleming, Suryakant Mishra, Kakali Santra, Francesco Tassinari, Sidney R. Cohen, Tapan Kumar Das, Yutao Sang, Deb K. Bhowmick, Cynthia J. Burrows^*, Yossi Paltiel^*, Ron Naaman^*

^*Corresponding author for this work

Department of Applied Physics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

Original language	English
Article number	101157
Journal	Cell Reports Physical Science
Volume	3
Issue number	12
DOIs	https://doi.org/10.1016/j.xcrp.2022.101157
State	Published - 21 Dec 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

CISS effect
DNA damage
atomic force microscope-based single-molecule force spectroscopy
chirality
double-stranded DNA
guanine oxidative damage
spin exchange
spin in biology

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title = "The role of electrons{\textquoteright} spin in DNA oxidative damage recognition",

abstract = "Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.",

keywords = "CISS effect, DNA damage, atomic force microscope-based single-molecule force spectroscopy, chirality, double-stranded DNA, guanine oxidative damage, spin exchange, spin in biology",

author = "Qirong Zhu and Yael Kapon and Fleming, {Aaron M.} and Suryakant Mishra and Kakali Santra and Francesco Tassinari and Cohen, {Sidney R.} and Das, {Tapan Kumar} and Yutao Sang and Bhowmick, {Deb K.} and Burrows, {Cynthia J.} and Yossi Paltiel and Ron Naaman",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = dec,

day = "21",

doi = "10.1016/j.xcrp.2022.101157",

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T1 - The role of electrons’ spin in DNA oxidative damage recognition

AU - Zhu, Qirong

AU - Kapon, Yael

AU - Fleming, Aaron M.

AU - Mishra, Suryakant

AU - Santra, Kakali

AU - Tassinari, Francesco

AU - Cohen, Sidney R.

AU - Das, Tapan Kumar

AU - Sang, Yutao

AU - Bhowmick, Deb K.

AU - Burrows, Cynthia J.

AU - Paltiel, Yossi

AU - Naaman, Ron

PY - 2022/12/21

Y1 - 2022/12/21

N2 - Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

AB - Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

KW - CISS effect

KW - DNA damage

KW - atomic force microscope-based single-molecule force spectroscopy

KW - chirality

KW - double-stranded DNA

KW - guanine oxidative damage

KW - spin exchange

KW - spin in biology

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JO - Cell Reports Physical Science

JF - Cell Reports Physical Science

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M1 - 101157

ER -

The role of electrons’ spin in DNA oxidative damage recognition

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Keywords

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