The role of electrons’ spin in DNA oxidative damage recognition

Qirong Zhu, Yael Kapon, Aaron M. Fleming, Suryakant Mishra, Kakali Santra, Francesco Tassinari, Sidney R. Cohen, Tapan Kumar Das, Yutao Sang, Deb K. Bhowmick, Cynthia J. Burrows*, Yossi Paltiel*, Ron Naaman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG) is one of the most common forms of DNA oxidative damage found in human cells. Although this damage is prevalent in many disease states, it only marginally influences the structure and stability of double-stranded DNA (dsDNA). Therefore, it is a challenge to establish the mechanism by which this damage is detected by repair enzymes. We investigated the position-dependent effect of the damage on the interactions between dsDNA and oligopeptides using atomic force microscopy. The results were confirmed by monitoring the spin and location-dependent polarizability of the damaged DNA, applying a Hall device. The observations suggest that the interaction of peptide with DNA depends on oxidative damage in the DNA and on its location relative to the point of contact between the peptide and the DNA. Hence, a remote search mechanism for damage in DNA is possible.

Original languageAmerican English
Article number101157
JournalCell Reports Physical Science
Volume3
Issue number12
DOIs
StatePublished - 21 Dec 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

  • CISS effect
  • DNA damage
  • atomic force microscope-based single-molecule force spectroscopy
  • chirality
  • double-stranded DNA
  • guanine oxidative damage
  • spin exchange
  • spin in biology

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