TY - JOUR
T1 - The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb
AU - Reshef, Ronen
AU - Kudryavitskaya, Elena
AU - Shani-Narkiss, Haran
AU - Isaacson, Batya
AU - Rimmerman, Neta
AU - Mizrahi, Adi
AU - Yirmiya, Raz
N1 - Publisher Copyright:
© Reshef et al.
PY - 2017/12/18
Y1 - 2017/12/18
N2 - Microglia play important roles in perinatal neuro-and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient (Cx3cr1-/-) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In Cx3cr1-/-mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between Cx3cr1-deficient microglia and abGCs’ dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.
AB - Microglia play important roles in perinatal neuro-and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient (Cx3cr1-/-) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In Cx3cr1-/-mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between Cx3cr1-deficient microglia and abGCs’ dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.
UR - http://www.scopus.com/inward/record.url?scp=85040925690&partnerID=8YFLogxK
U2 - 10.7554/eLife.30809
DO - 10.7554/eLife.30809
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 29251592
AN - SCOPUS:85040925690
SN - 2050-084X
VL - 6
JO - eLife
JF - eLife
M1 - e30809
ER -