TY - JOUR
T1 - The semisynthesis of nucleolar human selenoprotein H
AU - Dardashti, Rebecca Notis
AU - Laps, Shay
AU - Gichtin, Jacob S.
AU - Metanis, Norman
N1 - Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/10/24
Y1 - 2023/10/24
N2 - The human selenoprotein H is the only selenocysteine-containing protein that is located in the cell's nucleolus. In vivo studies have suggested that it plays some role in DNA binding, consumption of reactive oxygen species, and may serve as a safeguard against cancers. However, the protein has never been isolated and, as a result, not yet fully characterized. Here, we used a semi-synthetic approach to obtain the full selenoprotein H with a S43T mutation. Using biolayer interferometry, we also show that the Cys-containing mutant of selenoprotein H is capable of binding DNA with sub-micromolar affinity. Employing state-of-the-art expressed protein ligation (EPL), our devised semi-synthetic approach can be utilized for the production of numerous, hard-to-obtain proteins of biological and therapeutic relevance.
AB - The human selenoprotein H is the only selenocysteine-containing protein that is located in the cell's nucleolus. In vivo studies have suggested that it plays some role in DNA binding, consumption of reactive oxygen species, and may serve as a safeguard against cancers. However, the protein has never been isolated and, as a result, not yet fully characterized. Here, we used a semi-synthetic approach to obtain the full selenoprotein H with a S43T mutation. Using biolayer interferometry, we also show that the Cys-containing mutant of selenoprotein H is capable of binding DNA with sub-micromolar affinity. Employing state-of-the-art expressed protein ligation (EPL), our devised semi-synthetic approach can be utilized for the production of numerous, hard-to-obtain proteins of biological and therapeutic relevance.
UR - http://www.scopus.com/inward/record.url?scp=85176092724&partnerID=8YFLogxK
U2 - 10.1039/d3sc03059h
DO - 10.1039/d3sc03059h
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C2 - 38020378
AN - SCOPUS:85176092724
SN - 2041-6520
VL - 14
SP - 12723
EP - 12729
JO - Chemical Science
JF - Chemical Science
IS - 44
ER -