The Sensitivity of Phosphocholine 13C Chemical Shifts to pH

Ayelet Gamliel, Netanel Chendler, J. Moshe Gomori, Jacob Sosna, Rachel Katz-Brull*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Phosphocholine is a useful magnetic resonance (MR) biomarker for cancer. 13C hyperpolarized MR is likely to enhance the detection of this compound by orders of magnitude. The ability to differentiate phosphocholine from its precursor, choline, in live tissues, is unique to MR and depends on the 13C chemical shifts. The dependence of phosphocholine 13C chemical shifts on pH was investigated. We found that under viable physiological conditions of pH > 7, the chemical shift of phosphocholine 13C is stable and the chemical shift distance from the choline 13C signals is preserved. Under acidic intracellular conditions of 6.0 < pH < 7, the pH could be sensed by the phosphocholine 13C chemical shifts. Crucial and damaging pH changes to values lower than 6.0 could be sensed at very high sensitivity. The highest precision for pH determination is obtained when considering the chemical shift difference between positions 1 and 2 of phosphocholine. The solution composition of human plasma did not affect the response of phosphocholine 13C chemical shifts to pH.

Original languageAmerican English
Pages (from-to)111-120
Number of pages10
JournalApplied Magnetic Resonance
Volume47
Issue number1
DOIs
StatePublished - 1 Jan 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015, Springer-Verlag Wien.

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