The Sensitivity of Phosphocholine 13C Chemical Shifts to pH

Ayelet Gamliel; Netanel Chendler; J. Moshe Gomori; Jacob Sosna; Rachel Katz-Brull

doi:10.1007/s00723-015-0734-8

The Sensitivity of Phosphocholine ¹³C Chemical Shifts to pH

Ayelet Gamliel, Netanel Chendler, J. Moshe Gomori, Jacob Sosna, Rachel Katz-Brull^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Phosphocholine is a useful magnetic resonance (MR) biomarker for cancer. ¹³C hyperpolarized MR is likely to enhance the detection of this compound by orders of magnitude. The ability to differentiate phosphocholine from its precursor, choline, in live tissues, is unique to MR and depends on the ¹³C chemical shifts. The dependence of phosphocholine ¹³C chemical shifts on pH was investigated. We found that under viable physiological conditions of pH > 7, the chemical shift of phosphocholine ¹³C is stable and the chemical shift distance from the choline ¹³C signals is preserved. Under acidic intracellular conditions of 6.0 < pH < 7, the pH could be sensed by the phosphocholine ¹³C chemical shifts. Crucial and damaging pH changes to values lower than 6.0 could be sensed at very high sensitivity. The highest precision for pH determination is obtained when considering the chemical shift difference between positions 1 and 2 of phosphocholine. The solution composition of human plasma did not affect the response of phosphocholine ¹³C chemical shifts to pH.

Original language	American English
Pages (from-to)	111-120
Number of pages	10
Journal	Applied Magnetic Resonance
Volume	47
Issue number	1
DOIs	https://doi.org/10.1007/s00723-015-0734-8
State	Published - 1 Jan 2016
Externally published	Yes

Bibliographical note

Funding Information:
This work was funded by the Israel Science Foundation (Grant No. 284/10) and the European Research Council (Grant No. 338040).

Publisher Copyright:
© 2015, Springer-Verlag Wien.

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10.1007/s00723-015-0734-8

Cite this

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title = "The Sensitivity of Phosphocholine 13C Chemical Shifts to pH",

abstract = "Phosphocholine is a useful magnetic resonance (MR) biomarker for cancer. 13C hyperpolarized MR is likely to enhance the detection of this compound by orders of magnitude. The ability to differentiate phosphocholine from its precursor, choline, in live tissues, is unique to MR and depends on the 13C chemical shifts. The dependence of phosphocholine 13C chemical shifts on pH was investigated. We found that under viable physiological conditions of pH > 7, the chemical shift of phosphocholine 13C is stable and the chemical shift distance from the choline 13C signals is preserved. Under acidic intracellular conditions of 6.0 < pH < 7, the pH could be sensed by the phosphocholine 13C chemical shifts. Crucial and damaging pH changes to values lower than 6.0 could be sensed at very high sensitivity. The highest precision for pH determination is obtained when considering the chemical shift difference between positions 1 and 2 of phosphocholine. The solution composition of human plasma did not affect the response of phosphocholine 13C chemical shifts to pH.",

author = "Ayelet Gamliel and Netanel Chendler and {Moshe Gomori}, J. and Jacob Sosna and Rachel Katz-Brull",

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AU - Gamliel, Ayelet

AU - Chendler, Netanel

AU - Moshe Gomori, J.

AU - Sosna, Jacob

AU - Katz-Brull, Rachel

N1 - Funding Information: This work was funded by the Israel Science Foundation (Grant No. 284/10) and the European Research Council (Grant No. 338040). Publisher Copyright: © 2015, Springer-Verlag Wien.

PY - 2016/1/1

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N2 - Phosphocholine is a useful magnetic resonance (MR) biomarker for cancer. 13C hyperpolarized MR is likely to enhance the detection of this compound by orders of magnitude. The ability to differentiate phosphocholine from its precursor, choline, in live tissues, is unique to MR and depends on the 13C chemical shifts. The dependence of phosphocholine 13C chemical shifts on pH was investigated. We found that under viable physiological conditions of pH > 7, the chemical shift of phosphocholine 13C is stable and the chemical shift distance from the choline 13C signals is preserved. Under acidic intracellular conditions of 6.0 < pH < 7, the pH could be sensed by the phosphocholine 13C chemical shifts. Crucial and damaging pH changes to values lower than 6.0 could be sensed at very high sensitivity. The highest precision for pH determination is obtained when considering the chemical shift difference between positions 1 and 2 of phosphocholine. The solution composition of human plasma did not affect the response of phosphocholine 13C chemical shifts to pH.

AB - Phosphocholine is a useful magnetic resonance (MR) biomarker for cancer. 13C hyperpolarized MR is likely to enhance the detection of this compound by orders of magnitude. The ability to differentiate phosphocholine from its precursor, choline, in live tissues, is unique to MR and depends on the 13C chemical shifts. The dependence of phosphocholine 13C chemical shifts on pH was investigated. We found that under viable physiological conditions of pH > 7, the chemical shift of phosphocholine 13C is stable and the chemical shift distance from the choline 13C signals is preserved. Under acidic intracellular conditions of 6.0 < pH < 7, the pH could be sensed by the phosphocholine 13C chemical shifts. Crucial and damaging pH changes to values lower than 6.0 could be sensed at very high sensitivity. The highest precision for pH determination is obtained when considering the chemical shift difference between positions 1 and 2 of phosphocholine. The solution composition of human plasma did not affect the response of phosphocholine 13C chemical shifts to pH.

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