The separation of 2′-5′ dinucleoside monophosphates from the corresponding 3′-5′ isomers on a DEAE-Sephadex A-25 column

The separation of 2′-5′ dinucleoside monophosphates from their corresponding 3′-5′ isomers on a DEAE-Sephadex A-25 (bicarbonate form) column is described. The column is loaded with a mixture of 2′(3′)-5′ dinucleoside monophosphate and eluted with a linear gradient of ammonium bicarbonate (from 0.02 to 0.15 M. The two isomers come out in two different peaks, and the nucleotidic material is isolated by lyophylization.