The structure, composition and mechanical properties of the skeleton of the naked mole-rat (Heterocephalus glaber)

The naked mole-rat (NMR) is a small rodent with a remarkable array of properties, such as unique physiology, extremely long life-span and unusual social life. However, very little is known regarding its skeleton. The aim of this study was to describe the structure, composition and mechanical properties in an ontogenetic series of naked mole-rat bones. Since common small rodents like mice and rats have an unusual structure of cortical bone, which includes a central region of non-lamellar (disordered) bone, mineralized cartilaginous islands and total lack of remodeling, this study could also determine if these are features of all small rodents. Sixty-one NMRs were included in the study and were divided into the following four age groups: 0–0.5 years old (n = 17), 0.5–3 years old (n = 25), 3–10 years old (n = 13), and >10 years (n = 6). Femora, vertebrae and mandibulae were examined using micro-CT, light microscopy, polarized light microscopy and scanning electron microscopy, thermogravimetric analysis was used to determine their dry ash content and their derived elastic modulus and hardness were determined using micro-indentation. Our findings show that NMR bones are similar in composition and mechanical properties to those of other small rodents. However, in contrast to other small rodents, the cortical bone of NMRs is entirely circumferential-lamellar and lacks mineralized cartilage islands. Furthermore, despite their long life-span, their bones did not show evidence of remodeling at any of the age groups, thus proving that lack of cortical remodeling in small rodents is not caused by their short life-span, but characteristic of this order of mammals.