The structure of the human liver-type phosphofructokinase gene

A. Elson; D. Levanon; M. Brandeis; N. Dafni; Y. Bernstein; E. Danciger; Y. Groner

doi:10.1016/0888-7543(90)90517-X

The structure of the human liver-type phosphofructokinase gene

A. Elson, D. Levanon, M. Brandeis, N. Dafni, Y. Bernstein, E. Danciger, Y. Groner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We have isolated the gene for the human liver-type phosphofructokinase, from upstream to the 5′ mRNA terminus to beyond the polyadenylation site. The gene is at least 28 kb long and is divided into 22 exons; it contains conventional splice-junction sequences and one polyadenylation signal. Exons and introns are quite rich in G and C residues; some 60% of all nucleotides are either G or C. Five possible sites of polymorphism have been found. The gene structure reveals no signs of internal similarities despite protein sequence evidence which suggests that the PFK molecule is divided into two similar halves. The structure and organization of the human liver-type PFK gene are shown to be extremely similar to those of the rabbit muscle-type PFK.

Original language	English
Pages (from-to)	47-56
Number of pages	10
Journal	Genomics
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/0888-7543(90)90517-X
State	Published - May 1990
Externally published	Yes

Bibliographical note

Funding Information:
We thank Celina Gross for typing the manuscript. This work was supported by grants from the National Institutes of Health HD21229, the Minerva Foundation (Munich) FRG, and the Weizmann Institute’s Leo and Julia Forcheimer Center of Molecular Genetics and the Gudelsky Foundation.

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title = "The structure of the human liver-type phosphofructokinase gene",

abstract = "We have isolated the gene for the human liver-type phosphofructokinase, from upstream to the 5′ mRNA terminus to beyond the polyadenylation site. The gene is at least 28 kb long and is divided into 22 exons; it contains conventional splice-junction sequences and one polyadenylation signal. Exons and introns are quite rich in G and C residues; some 60% of all nucleotides are either G or C. Five possible sites of polymorphism have been found. The gene structure reveals no signs of internal similarities despite protein sequence evidence which suggests that the PFK molecule is divided into two similar halves. The structure and organization of the human liver-type PFK gene are shown to be extremely similar to those of the rabbit muscle-type PFK.",

author = "A. Elson and D. Levanon and M. Brandeis and N. Dafni and Y. Bernstein and E. Danciger and Y. Groner",

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AU - Levanon, D.

AU - Brandeis, M.

AU - Dafni, N.

AU - Bernstein, Y.

AU - Danciger, E.

AU - Groner, Y.

N1 - Funding Information: We thank Celina Gross for typing the manuscript. This work was supported by grants from the National Institutes of Health HD21229, the Minerva Foundation (Munich) FRG, and the Weizmann Institute’s Leo and Julia Forcheimer Center of Molecular Genetics and the Gudelsky Foundation.

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AB - We have isolated the gene for the human liver-type phosphofructokinase, from upstream to the 5′ mRNA terminus to beyond the polyadenylation site. The gene is at least 28 kb long and is divided into 22 exons; it contains conventional splice-junction sequences and one polyadenylation signal. Exons and introns are quite rich in G and C residues; some 60% of all nucleotides are either G or C. Five possible sites of polymorphism have been found. The gene structure reveals no signs of internal similarities despite protein sequence evidence which suggests that the PFK molecule is divided into two similar halves. The structure and organization of the human liver-type PFK gene are shown to be extremely similar to those of the rabbit muscle-type PFK.

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The structure of the human liver-type phosphofructokinase gene

Abstract

Bibliographical note

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