The structure of the stalk surface layer of a brine pond microorganism: correlation averaging applied to a double layered lattice structure

Martin Kessel*, Michael Radermacher, Joachim Frank

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The surface layer of the stalk of a prosthecate halophilic microorganism is a periodic array (space group p3ml) comprised of electron dense trimers with a centre to centre spacing of 9.0nm. The structure is reminiscent of E. coli porin. We have demonstrated that the method of correlation averaging can be effectively used to separate overlapping lattices, and that this results in a higher fidelity reconstruction, when compared to the established method of Fourier filtration. Two statistical methods are used to determine the resolution of the correlation average as a function of the number of ‘windows’ averaged, (i) The phase residual of spatial frequencies in Fourier space is computed between independently obtained subaverages, and (ii) a new method of Q factor analysis examines the cumulative vector sum in Fourier space as a function of the number of windows averaged. Both methods give a resolution of 1/2.1−nm. 1985 Blackwell Science Ltd

Original languageEnglish
Pages (from-to)63-74
Number of pages12
JournalJournal of Microscopy
Volume139
Issue number1
DOIs
StatePublished - Jul 1985
Externally publishedYes

Keywords

  • bacterial cell wall
  • correlation averaging
  • overlapping lattices
  • phase residual
  • Prosthecate microorganism
  • Q‐factor
  • stalk surface reconstruction

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