TY - JOUR
T1 - The structure of the stalk surface layer of a brine pond microorganism
T2 - correlation averaging applied to a double layered lattice structure
AU - Kessel, Martin
AU - Radermacher, Michael
AU - Frank, Joachim
PY - 1985/7
Y1 - 1985/7
N2 - The surface layer of the stalk of a prosthecate halophilic microorganism is a periodic array (space group p3ml) comprised of electron dense trimers with a centre to centre spacing of 9.0nm. The structure is reminiscent of E. coli porin. We have demonstrated that the method of correlation averaging can be effectively used to separate overlapping lattices, and that this results in a higher fidelity reconstruction, when compared to the established method of Fourier filtration. Two statistical methods are used to determine the resolution of the correlation average as a function of the number of ‘windows’ averaged, (i) The phase residual of spatial frequencies in Fourier space is computed between independently obtained subaverages, and (ii) a new method of Q factor analysis examines the cumulative vector sum in Fourier space as a function of the number of windows averaged. Both methods give a resolution of 1/2.1−nm. 1985 Blackwell Science Ltd
AB - The surface layer of the stalk of a prosthecate halophilic microorganism is a periodic array (space group p3ml) comprised of electron dense trimers with a centre to centre spacing of 9.0nm. The structure is reminiscent of E. coli porin. We have demonstrated that the method of correlation averaging can be effectively used to separate overlapping lattices, and that this results in a higher fidelity reconstruction, when compared to the established method of Fourier filtration. Two statistical methods are used to determine the resolution of the correlation average as a function of the number of ‘windows’ averaged, (i) The phase residual of spatial frequencies in Fourier space is computed between independently obtained subaverages, and (ii) a new method of Q factor analysis examines the cumulative vector sum in Fourier space as a function of the number of windows averaged. Both methods give a resolution of 1/2.1−nm. 1985 Blackwell Science Ltd
KW - bacterial cell wall
KW - correlation averaging
KW - overlapping lattices
KW - phase residual
KW - Prosthecate microorganism
KW - Q‐factor
KW - stalk surface reconstruction
UR - http://www.scopus.com/inward/record.url?scp=84985269049&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2818.1985.tb04662.x
DO - 10.1111/j.1365-2818.1985.tb04662.x
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C2 - 4046014
AN - SCOPUS:84985269049
SN - 0022-2720
VL - 139
SP - 63
EP - 74
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 1
ER -