TY - JOUR
T1 - The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin
AU - Martens, Nele
AU - Wery, Maxime
AU - Wang, Ping
AU - Braet, Filip
AU - Gertler, Arieh
AU - Hooghe, Robert
AU - Vandenhaute, Jean
AU - Hooghe-Peters, Elisabeth L.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - To understand the function of the suppressor of cytokine signaling (SOCS)-7, we have looked for proteins interacting with SOCS-7 in a stringent yeast two-hybrid screen of a human leukocyte cDNA-library. We identified the cytoskeletal molecule vinexin as a partner interacting with SOCS-7. Tests with deletion mutants of SOCS-7 demonstrated that a central region of the molecule containing several proline-rich regions, N-terminal to the SH2 domain, was responsible for the binding to vinexin. It is thus likely that one of the SH3 domains of vinexin interacts with a poly-proline region of SOCS-7. The interaction with vinexin was confirmed biochemically as vinexin-α was co-precipitated with SOCS-7. Confocal laser-scanning microscopy in HEK293T, MCF-7, and 3T3-L1 cells showed that part of the transfected SOCS-7-green fluorescent protein (GFP) molecules merged with vinexin and with actin. Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton.
AB - To understand the function of the suppressor of cytokine signaling (SOCS)-7, we have looked for proteins interacting with SOCS-7 in a stringent yeast two-hybrid screen of a human leukocyte cDNA-library. We identified the cytoskeletal molecule vinexin as a partner interacting with SOCS-7. Tests with deletion mutants of SOCS-7 demonstrated that a central region of the molecule containing several proline-rich regions, N-terminal to the SH2 domain, was responsible for the binding to vinexin. It is thus likely that one of the SH3 domains of vinexin interacts with a poly-proline region of SOCS-7. The interaction with vinexin was confirmed biochemically as vinexin-α was co-precipitated with SOCS-7. Confocal laser-scanning microscopy in HEK293T, MCF-7, and 3T3-L1 cells showed that part of the transfected SOCS-7-green fluorescent protein (GFP) molecules merged with vinexin and with actin. Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton.
KW - Actin
KW - Nck
KW - Signal transduction
KW - Vinculin
KW - Vinexin
UR - http://www.scopus.com/inward/record.url?scp=3042823699&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2004.04.002
DO - 10.1016/j.yexcr.2004.04.002
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C2 - 15242778
AN - SCOPUS:3042823699
SN - 0014-4827
VL - 298
SP - 239
EP - 248
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -