Abstract
The length of the premeiotic S phase in individual cells of a homothallic strain of Saccharomyces cerevisiae was determined by pulse-labelling synchronously sporulating cultures with [3H]adenine and following changes in the frequency of cells in S by DNA-specific whole cell autoradiography. The average S phase was found to be at least 2-3 times as long as in mitotic diploids, and it was concluded that activation of replication origins in meiosis is considerably staggered. The timing of S in relation to other meiotic milestones was established by counting different nuclear morphologies visualised by DAPI-fluorescent staining. This allowed tentative identification of pachytene nuclei as well as first and second nuclear divisions.
Original language | English |
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Pages (from-to) | 209-217 |
Number of pages | 9 |
Journal | Experimental Cell Research |
Volume | 145 |
Issue number | 1 |
DOIs | |
State | Published - 15 Apr 1983 |