The timing of the S phase and other nuclear events in yeast meiosis

D. H. Williamson*, L. H. Johnston, D. J. Fennell, G. Simchen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The length of the premeiotic S phase in individual cells of a homothallic strain of Saccharomyces cerevisiae was determined by pulse-labelling synchronously sporulating cultures with [3H]adenine and following changes in the frequency of cells in S by DNA-specific whole cell autoradiography. The average S phase was found to be at least 2-3 times as long as in mitotic diploids, and it was concluded that activation of replication origins in meiosis is considerably staggered. The timing of S in relation to other meiotic milestones was established by counting different nuclear morphologies visualised by DAPI-fluorescent staining. This allowed tentative identification of pachytene nuclei as well as first and second nuclear divisions.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalExperimental Cell Research
Volume145
Issue number1
DOIs
StatePublished - 15 Apr 1983

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