The toxicity of T-2 toxin and diacetoxyscirpenol in combination for broiler chickens

T-2 toxin and diacetoxyscirpenol, two naturally occurring trichothecene mycotoxins, were dissolved in dimethylsulphoxide:saline (1:9, v/v) and administered separately and in combinations to 7-day-old male broiler chickens. The 72-hr single oral dose LD₅₀ for T-2 toxin was 4·0 mg/kg body weight and for diacetoxyscirpenol, it was 5·0 mg/kg. T-2 toxin administered as 14 daily oral doses had an LD₅₀ of 2·90 mg/kg, and diacetoxyscirpenol had an LD₅₀ of 4·15 mg/kg. Combinations of the toxins caused additive lethal effects in both the single- and multiple-dose tests.