TY - JOUR
T1 - The trip adviser guide to the protein science world
T2 - A proposal to improve the awareness concerning the quality of recombinant proteins
AU - Lebendiker, Mario
AU - Danieli, Tsafi
AU - De Marco, Ario
N1 - Publisher Copyright:
© 2014 Lebendiker et al.;.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a "quality control" ladder, where the more information given, will lead to a higher ranking of the article. This "quality control" stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research.
AB - In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a "quality control" ladder, where the more information given, will lead to a higher ranking of the article. This "quality control" stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research.
KW - Data annotation
KW - Data reproducibility
KW - Protein quality
UR - http://www.scopus.com/inward/record.url?scp=84907966299&partnerID=8YFLogxK
U2 - 10.1186/1756-0500-7-585
DO - 10.1186/1756-0500-7-585
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C2 - 25178166
AN - SCOPUS:84907966299
SN - 1756-0500
VL - 7
JO - BMC Research Notes
JF - BMC Research Notes
IS - 1
M1 - 585
ER -