TY - JOUR
T1 - The use of cyclic voltammetry for the evaluation of antioxidant capacity
AU - Chevion, Shlomit
AU - Roberts, Matthew A.
AU - Chevion, Mordechai
PY - 2000/3/15
Y1 - 2000/3/15
N2 - Low-molecular weight antioxidants (LMWAs) play a major role in protecting biological systems against reactive oxygen-derived species and reflect the antioxidant capacity of the system. Cyclic voltammetry (CV), shown to be convenient methodology, has been validated for quantitation of the LMWA capacity of blood plasma, tissue homogenates, and plant extracts. Analysis of the CV tracing yields the values of (i) the biological oxidation potential, E and E(1/2), which relate to the nature of the specific molecule(s); (ii) the intensity (Ia) of the anodic current; and (iii) the area of the anodic wave (S). Both Ia and S relate to the concentration of the molecule(s). LMWA components of human plasma and animal tissues were identified and further validated by reconstruction of the CV tracing and by high-performance liquid chromatography-electrochemical detection. To reflect the oxidative stress status, the use of an additional parameter, R, has been proposed. R represents the level (%) of oxidized ascorbate (compared with total ascorbate) and is measured by high-performance liquid chromatography-electrochemical detection. All these parameters were monitored in healthy human subjects as well as in chronic (diabetes mellitus) and acute care patients (subjected to total body irradiation before bone marrow transplantation). The electroanalytical methodologies presented here could be widely employed for rapid evaluation of the status of subjects (in health and disease) for monitoring of their response to treatment and/or nutritional supplementation as well as for screening of specific populations. Copyright (C) 2000 Elsevier Science Inc.
AB - Low-molecular weight antioxidants (LMWAs) play a major role in protecting biological systems against reactive oxygen-derived species and reflect the antioxidant capacity of the system. Cyclic voltammetry (CV), shown to be convenient methodology, has been validated for quantitation of the LMWA capacity of blood plasma, tissue homogenates, and plant extracts. Analysis of the CV tracing yields the values of (i) the biological oxidation potential, E and E(1/2), which relate to the nature of the specific molecule(s); (ii) the intensity (Ia) of the anodic current; and (iii) the area of the anodic wave (S). Both Ia and S relate to the concentration of the molecule(s). LMWA components of human plasma and animal tissues were identified and further validated by reconstruction of the CV tracing and by high-performance liquid chromatography-electrochemical detection. To reflect the oxidative stress status, the use of an additional parameter, R, has been proposed. R represents the level (%) of oxidized ascorbate (compared with total ascorbate) and is measured by high-performance liquid chromatography-electrochemical detection. All these parameters were monitored in healthy human subjects as well as in chronic (diabetes mellitus) and acute care patients (subjected to total body irradiation before bone marrow transplantation). The electroanalytical methodologies presented here could be widely employed for rapid evaluation of the status of subjects (in health and disease) for monitoring of their response to treatment and/or nutritional supplementation as well as for screening of specific populations. Copyright (C) 2000 Elsevier Science Inc.
KW - Ascorbate
KW - Cyclic voltammetry
KW - Dehydroascorbate
KW - Diabetes
KW - Free radical
KW - Low-molecular weight antioxidant
KW - Plant antioxidants
KW - Total antioxidant capacity
KW - Total body irradiation
UR - http://www.scopus.com/inward/record.url?scp=0034654255&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(00)00178-7
DO - 10.1016/S0891-5849(00)00178-7
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C2 - 10802216
AN - SCOPUS:0034654255
SN - 0891-5849
VL - 28
SP - 860
EP - 870
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 6
ER -