The Use of Fluorescent Proteins to Analyze Circadian Rhythms

Ekaterina Shor, Miriam Hassidim, Rachel M. Green

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.

Original languageAmerican English
Pages (from-to)209-213
Number of pages5
JournalMethods in Molecular Biology
Volume1158
DOIs
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

  • Arabidopsis
  • Circadian
  • Confocal
  • Fluorescent
  • Nuclear
  • Protein

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