The use of gapmers for in vivo suppression of hepatic mRNA targets

David S. Greenberg; Yonat Tzur; Hermona Soreq

doi:10.1007/978-1-0716-0771-8_13

The use of gapmers for in vivo suppression of hepatic mRNA targets

David S. Greenberg, Yonat Tzur, Hermona Soreq^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

This chapter describes the use of locked nucleic acid (LNA) GapmeRs for the in vivo knockdown of specific mRNAs in the mouse liver and phenotype analysis. LNA GapmeRs may be tested for efficacy by transfection in cultured cells. They are delivered into mice in vivo by intravenous tail injection.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	177-184
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-0716-0771-8_13
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2176
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

Keywords

Diet-induced obese mice
In vivo knockdown
LNA gapmers
Liver mRNA
Steatosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-0771-8_13

Cite this

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The use of gapmers for in vivo suppression of hepatic mRNA targets

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

Access to Document

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