TY - JOUR
T1 - The Use of Lyophilized Schistosoma Mansoni Eggs as Antigenic Particles in a Radioimmunoassay
AU - Hamburger, J.
AU - Moscovici, H.
AU - Lustigman, S.
PY - 1983/12
Y1 - 1983/12
N2 - Lyophilized eggs of Schistosoma mansoni, when incubated briefly with serum from infected mice, bind antibodies, as made evident by subsequent binding of flurorescein labelled anti-IgG or 125I-labelled Protein A. On the basis of these findings, a radioimmunoassay was devised which employs whole lyophilized eggs (500 or 250 eggs/serum sample) as antigenic particles and 125I-labelled Protein A as a probe for antibody binding. Only 10/d of serum are required to obtain 90% of the maximal binding. Kinetic studies indicated that 70% of the maximal seropositivity develops in mice between five and six weeks after a light infection, reaches a maximum at eight weeks and fluctuates around a high plateau thereafter. Pre-incubation of the test serum with soluble egg antigen (SEA) considerably inhibits antibody binding to the eggs, suggesting that SEA-like antigens participate in the reaction.
AB - Lyophilized eggs of Schistosoma mansoni, when incubated briefly with serum from infected mice, bind antibodies, as made evident by subsequent binding of flurorescein labelled anti-IgG or 125I-labelled Protein A. On the basis of these findings, a radioimmunoassay was devised which employs whole lyophilized eggs (500 or 250 eggs/serum sample) as antigenic particles and 125I-labelled Protein A as a probe for antibody binding. Only 10/d of serum are required to obtain 90% of the maximal binding. Kinetic studies indicated that 70% of the maximal seropositivity develops in mice between five and six weeks after a light infection, reaches a maximum at eight weeks and fluctuates around a high plateau thereafter. Pre-incubation of the test serum with soluble egg antigen (SEA) considerably inhibits antibody binding to the eggs, suggesting that SEA-like antigens participate in the reaction.
UR - http://www.scopus.com/inward/record.url?scp=0020999877&partnerID=8YFLogxK
U2 - 10.1017/S0022149X00010993
DO - 10.1017/S0022149X00010993
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C2 - 6366044
AN - SCOPUS:0020999877
SN - 0022-149X
VL - 57
SP - 305
EP - 312
JO - Journal of Helminthology
JF - Journal of Helminthology
IS - 4
ER -