The use of the hydrodynamic HBV animal model to study HBV biology and anti-viral therapy

Mali Ketzinel-Gilad; Arie Zauberman; Ofer Nussbaum; Yariv Shoshany; Ofer Ben-Moshe; Orit Pappo; Yaakov Felig; Ehud Ilan; Hanna Wald; Shlomo Dagan; Eithan Galun

doi:10.1016/j.hepres.2006.01.008

The use of the hydrodynamic HBV animal model to study HBV biology and anti-viral therapy

Mali Ketzinel-Gilad^*, Arie Zauberman, Ofer Nussbaum, Yariv Shoshany, Ofer Ben-Moshe, Orit Pappo, Yaakov Felig, Ehud Ilan, Hanna Wald, Shlomo Dagan, Eithan Galun

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A simple reproducible and versatile small animal model for hepatitis B virus (HBV) infection is still unavailable. We have generated a simple transient liver-targeted transgenic mouse. Hydrodynamics tail vein injection of a head-to-tail dimer of adw HBV genome (pHBVadwHTD) into immunocompetent mice generated HBsAg and HBeAg expression in both serum and hepatocytes, followed by seroconversion. The injection of pHBVadwHTD into SCID mice generated prolonged HBsAg and HBeAg antigenemia and HBV viremia. Our results demonstrate that hydrodynamic injection of naked DNA could support the generation of HBV particles. We used this model for the assessment of anti-viral agents. Administration of our human monoclonal antibodies, HBV-Ab17^XTL and HBV-Ab19^XTL, as well as Lamivudine (3TC) treatment suppressed HBV viremia. The model presented herein supports long and stable expression of HBV and will enable determination of various biological questions related to HBV life cycle, mutants and could enhance the development of anti-viral reagents.

Original language	English
Pages (from-to)	228-237
Number of pages	10
Journal	Hepatology Research
Volume	34
Issue number	4
DOIs	https://doi.org/10.1016/j.hepres.2006.01.008
State	Published - Apr 2006
Externally published	Yes

Bibliographical note

Funding Information:
The study was supported by a grant from XTL Biopharmaceuticals Ltd., the Israeli Ministry of Science and by the Horwitz, Blum and Grinspoon Foundation.

Keywords

Animal models
Hepatitis B virus infection
Hydrodynamic injection
Naked DNA
Transgenic mice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.hepres.2006.01.008

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title = "The use of the hydrodynamic HBV animal model to study HBV biology and anti-viral therapy",

abstract = "A simple reproducible and versatile small animal model for hepatitis B virus (HBV) infection is still unavailable. We have generated a simple transient liver-targeted transgenic mouse. Hydrodynamics tail vein injection of a head-to-tail dimer of adw HBV genome (pHBVadwHTD) into immunocompetent mice generated HBsAg and HBeAg expression in both serum and hepatocytes, followed by seroconversion. The injection of pHBVadwHTD into SCID mice generated prolonged HBsAg and HBeAg antigenemia and HBV viremia. Our results demonstrate that hydrodynamic injection of naked DNA could support the generation of HBV particles. We used this model for the assessment of anti-viral agents. Administration of our human monoclonal antibodies, HBV-Ab17XTL and HBV-Ab19XTL, as well as Lamivudine (3TC) treatment suppressed HBV viremia. The model presented herein supports long and stable expression of HBV and will enable determination of various biological questions related to HBV life cycle, mutants and could enhance the development of anti-viral reagents.",

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author = "Mali Ketzinel-Gilad and Arie Zauberman and Ofer Nussbaum and Yariv Shoshany and Ofer Ben-Moshe and Orit Pappo and Yaakov Felig and Ehud Ilan and Hanna Wald and Shlomo Dagan and Eithan Galun",

note = "Funding Information: The study was supported by a grant from XTL Biopharmaceuticals Ltd., the Israeli Ministry of Science and by the Horwitz, Blum and Grinspoon Foundation. ",

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AU - Ketzinel-Gilad, Mali

AU - Zauberman, Arie

AU - Nussbaum, Ofer

AU - Shoshany, Yariv

AU - Ben-Moshe, Ofer

AU - Pappo, Orit

AU - Felig, Yaakov

AU - Ilan, Ehud

AU - Wald, Hanna

AU - Dagan, Shlomo

AU - Galun, Eithan

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N2 - A simple reproducible and versatile small animal model for hepatitis B virus (HBV) infection is still unavailable. We have generated a simple transient liver-targeted transgenic mouse. Hydrodynamics tail vein injection of a head-to-tail dimer of adw HBV genome (pHBVadwHTD) into immunocompetent mice generated HBsAg and HBeAg expression in both serum and hepatocytes, followed by seroconversion. The injection of pHBVadwHTD into SCID mice generated prolonged HBsAg and HBeAg antigenemia and HBV viremia. Our results demonstrate that hydrodynamic injection of naked DNA could support the generation of HBV particles. We used this model for the assessment of anti-viral agents. Administration of our human monoclonal antibodies, HBV-Ab17XTL and HBV-Ab19XTL, as well as Lamivudine (3TC) treatment suppressed HBV viremia. The model presented herein supports long and stable expression of HBV and will enable determination of various biological questions related to HBV life cycle, mutants and could enhance the development of anti-viral reagents.

AB - A simple reproducible and versatile small animal model for hepatitis B virus (HBV) infection is still unavailable. We have generated a simple transient liver-targeted transgenic mouse. Hydrodynamics tail vein injection of a head-to-tail dimer of adw HBV genome (pHBVadwHTD) into immunocompetent mice generated HBsAg and HBeAg expression in both serum and hepatocytes, followed by seroconversion. The injection of pHBVadwHTD into SCID mice generated prolonged HBsAg and HBeAg antigenemia and HBV viremia. Our results demonstrate that hydrodynamic injection of naked DNA could support the generation of HBV particles. We used this model for the assessment of anti-viral agents. Administration of our human monoclonal antibodies, HBV-Ab17XTL and HBV-Ab19XTL, as well as Lamivudine (3TC) treatment suppressed HBV viremia. The model presented herein supports long and stable expression of HBV and will enable determination of various biological questions related to HBV life cycle, mutants and could enhance the development of anti-viral reagents.

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The use of the hydrodynamic HBV animal model to study HBV biology and anti-viral therapy

Abstract

Bibliographical note

Keywords

UN SDGs

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