TY - JOUR
T1 - The voltage-gated calcium channel functions as the molecular switch of synaptic transmission
AU - Atlas, Daphne
PY - 2013/6
Y1 - 2013/6
N2 - Transmitter release is a fast Ca2+-dependent process triggered in response to membrane depolarization. It involves two major calcium-binding proteins, the voltage-gated calcium channel (VGCC) and the vesicular protein synaptotagmin (syt1). Ca2+ binding triggers transmitter release with a time response of conformational changes that are too fast to be accounted for by Ca2+ binding to syt1. In contrast, conformation-triggered release, which engages Ca2+ binding to VGCC, better accounts for the fast rate of the release process. Here, we summarize findings obtained from heterologous expression systems, neuroendocrine cells, and reconstituted systems, which reveal the molecular mechanism by which Ca2+ binding to VGCC triggers exocytosis prior to Ca2+ entry into the cell. This review highlights the molecular aspects of an intramembrane signaling mechanism in which a signal is propagated from the channel transmembrane (TM) domain to the TM domain of syntaxin 1A to trigger transmitter release. It discusses fundamental problems of triggering transmitter release by syt1 and suggests a classification of docked vesicles that might explain synchronous transmitter release, spontaneous release, and facilitation of transmitter release.
AB - Transmitter release is a fast Ca2+-dependent process triggered in response to membrane depolarization. It involves two major calcium-binding proteins, the voltage-gated calcium channel (VGCC) and the vesicular protein synaptotagmin (syt1). Ca2+ binding triggers transmitter release with a time response of conformational changes that are too fast to be accounted for by Ca2+ binding to syt1. In contrast, conformation-triggered release, which engages Ca2+ binding to VGCC, better accounts for the fast rate of the release process. Here, we summarize findings obtained from heterologous expression systems, neuroendocrine cells, and reconstituted systems, which reveal the molecular mechanism by which Ca2+ binding to VGCC triggers exocytosis prior to Ca2+ entry into the cell. This review highlights the molecular aspects of an intramembrane signaling mechanism in which a signal is propagated from the channel transmembrane (TM) domain to the TM domain of syntaxin 1A to trigger transmitter release. It discusses fundamental problems of triggering transmitter release by syt1 and suggests a classification of docked vesicles that might explain synchronous transmitter release, spontaneous release, and facilitation of transmitter release.
KW - Amperometry
KW - Chromaffin cells
KW - Evoked secretion
KW - Excitosome
KW - Intramembrane signaling
KW - Lanthanides
UR - http://www.scopus.com/inward/record.url?scp=84878238928&partnerID=8YFLogxK
U2 - 10.1146/annurev-biochem-080411-121438
DO - 10.1146/annurev-biochem-080411-121438
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C2 - 23331239
AN - SCOPUS:84878238928
SN - 0066-4154
VL - 82
SP - 607
EP - 635
JO - Annual Review of Biochemistry
JF - Annual Review of Biochemistry
ER -