The Yeast ER-Intramembrane Protease Ypf1 Refines Nutrient Sensing by Regulating Transporter Abundance

Dönem Avci, Shai Fuchs, Bianca Schrul, Akio Fukumori, Michal Breker, Idan Frumkin, Chia Yi Chen, Martin L. Biniossek, Elisabeth Kremmer, Oliver Schilling, Harald Steiner, Maya Schuldiner*, Marius K. Lemberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Proteolysis by aspartyl intramembrane proteases such as presenilin and signal peptide peptidase (SPP) underlies many cellular processes in health and disease. Saccharomyces cerevisiae encodes a homolog that we named yeast presenilin fold 1 (Ypf1), which we verify to be an SPP-type protease that localizes to the endoplasmic reticulum (ER). Our work shows that Ypf1 functionally interacts with the ER-associated degradation (ERAD) factors Dfm1 and Doa10 to regulate the abundance of nutrient transporters by degradation. We demonstrate how this noncanonical branch of the ERAD pathway, which we termed "ERAD regulatory" (ERAD-R), responds to ligand-mediated sensing as a trigger. More generally, we show that Ypf1-mediated posttranslational regulation of plasma membrane transporters is indispensible for early sensing and adaptation to nutrient depletion. The combination of systematic analysis alongside mechanistic details uncovers a broad role of intramembrane proteolysis in regulating secretome dynamics.

Original languageAmerican English
Pages (from-to)630-640
Number of pages11
JournalMolecular Cell
Volume56
Issue number5
DOIs
StatePublished - 4 Dec 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Inc.

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