TY - JOUR
T1 - Theiler's murine encephalomyelitis virus group includes two distinct genetic subgroups that differ pathologically and biologically
AU - Lorch, Y.
AU - Friedmann, A.
AU - Lipton, H. L.
AU - Kotler, M.
PY - 1981
Y1 - 1981
N2 - The intracellular development and RNA composition of Theiler's murine encephalomyelitis virus (TMEV) isolates were determined by electron microscopy, sucrose gradient centrifugation, and RNase T1 fingerprinting. Replication of FA virus, a virulent strain of TMEV, was characterized by the appearance of viral crystalline arrays in the cytoplasm of infected cells. In contrast, cells infected with the less virulent isolates (WW, TO4, BeAn 8386, and Yale) showed no crystalline arrays; instead, virions were found to be arranged between two layers of membranes in the cytoplasm of infected cells. Analysis of the RNAs of TMEV isolates showed that the RNAs were single-stranded molecules having sedimentation coefficients of 35S. RNase T1 fingerprinting of TMEW RNA revealed that striking differences between the virulent and less virulent TMEV isolates existed. Moreover, base composition analysis of RNase T1-resistant oligonucleotides of two TMEV isolates which represented the two subgroups indicated that there were no substantial oligonucleotides common to both subgroups. Based on these findings and the known difference in virulence, we suggest that the TMEV group contains two genetically distinct subgroups of viruses.
AB - The intracellular development and RNA composition of Theiler's murine encephalomyelitis virus (TMEV) isolates were determined by electron microscopy, sucrose gradient centrifugation, and RNase T1 fingerprinting. Replication of FA virus, a virulent strain of TMEV, was characterized by the appearance of viral crystalline arrays in the cytoplasm of infected cells. In contrast, cells infected with the less virulent isolates (WW, TO4, BeAn 8386, and Yale) showed no crystalline arrays; instead, virions were found to be arranged between two layers of membranes in the cytoplasm of infected cells. Analysis of the RNAs of TMEV isolates showed that the RNAs were single-stranded molecules having sedimentation coefficients of 35S. RNase T1 fingerprinting of TMEW RNA revealed that striking differences between the virulent and less virulent TMEV isolates existed. Moreover, base composition analysis of RNase T1-resistant oligonucleotides of two TMEV isolates which represented the two subgroups indicated that there were no substantial oligonucleotides common to both subgroups. Based on these findings and the known difference in virulence, we suggest that the TMEV group contains two genetically distinct subgroups of viruses.
UR - http://www.scopus.com/inward/record.url?scp=0019848752&partnerID=8YFLogxK
U2 - 10.1128/jvi.40.2.560-567.1981
DO - 10.1128/jvi.40.2.560-567.1981
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C2 - 6275104
AN - SCOPUS:0019848752
SN - 0022-538X
VL - 40
SP - 560
EP - 567
JO - Journal of Virology
JF - Journal of Virology
IS - 2
ER -