TY - JOUR
T1 - Thermoanaerobacterium aciditolerans sp. nov., a moderate thermoacidophile from a Kamchatka hot spring
AU - Kublanov, I. V.
AU - Prokofeva, M. I.
AU - Kostrikina, N. A.
AU - Kolganova, T. V.
AU - Tourova, T. P.
AU - Wiegel, J.
AU - Bonch-Osmolovskaya, E. A.
PY - 2007/2
Y1 - 2007/2
N2 - An anaerobic, moderately thermoacidophilic bacterium, strain 761-119T was isolated from an acidic hot spring in the Orange Field of the Uzon Caldera (Kamchatka, far-eastern Russia). Cells were spore-forming, Gram-positive rods, possessing one polar flagellum. Growth of strain 761-119T was observed between 37 and 68 °C and in the pH20 °C range 3.2-7.1 No growth was observed within 5 days of incubation at or below 35 °C, and at or above 70 °C as well as at or below pH20 °C 2.8 and at or above pH20 °C 7.5. The optimal temperature and pH20 °C for growth were 55 °C and pH20 °C 5.7, respectively. A wide range of carbohydrates and polysaccharides were fermented, as well as peptides and proteinaceous substrates. The main products of glucose fermentation were acetate, ethanol, lactate, H2 and CO2. The DNA G+C content was 34 (±0.5) mol%. 16S rRNA gene sequence analysis indicated that strain 761-119T belonged to the genus Thermoanaerobacterium. The level of 16S rRNA gene sequence similarity with other Thermoanaerobacterium species was 86.5-97.8%, with the only moderately acidophilic member of this genus, Thermoanaerobacterium aotearoense, being one of its closest relatives. DNA-DNA hybridization with T aotearoense showed 33% relatedness. Thus, morphological (one polar flagellum) and physiological characteristics (lower pH limit of growth at pH20 °C 3.2 compared with T. aotearoense) and 16S rRNA gene sequence analyses revealed that strain 761-119T represents a novel species in the genus Thermoanaerobacterium, for which the name Thermoanaerobacterium aciditolerans sp. nov. is proposed, with the type strain 761-119T (= DSM 16487T = VKM B-2363T).
AB - An anaerobic, moderately thermoacidophilic bacterium, strain 761-119T was isolated from an acidic hot spring in the Orange Field of the Uzon Caldera (Kamchatka, far-eastern Russia). Cells were spore-forming, Gram-positive rods, possessing one polar flagellum. Growth of strain 761-119T was observed between 37 and 68 °C and in the pH20 °C range 3.2-7.1 No growth was observed within 5 days of incubation at or below 35 °C, and at or above 70 °C as well as at or below pH20 °C 2.8 and at or above pH20 °C 7.5. The optimal temperature and pH20 °C for growth were 55 °C and pH20 °C 5.7, respectively. A wide range of carbohydrates and polysaccharides were fermented, as well as peptides and proteinaceous substrates. The main products of glucose fermentation were acetate, ethanol, lactate, H2 and CO2. The DNA G+C content was 34 (±0.5) mol%. 16S rRNA gene sequence analysis indicated that strain 761-119T belonged to the genus Thermoanaerobacterium. The level of 16S rRNA gene sequence similarity with other Thermoanaerobacterium species was 86.5-97.8%, with the only moderately acidophilic member of this genus, Thermoanaerobacterium aotearoense, being one of its closest relatives. DNA-DNA hybridization with T aotearoense showed 33% relatedness. Thus, morphological (one polar flagellum) and physiological characteristics (lower pH limit of growth at pH20 °C 3.2 compared with T. aotearoense) and 16S rRNA gene sequence analyses revealed that strain 761-119T represents a novel species in the genus Thermoanaerobacterium, for which the name Thermoanaerobacterium aciditolerans sp. nov. is proposed, with the type strain 761-119T (= DSM 16487T = VKM B-2363T).
UR - http://www.scopus.com/inward/record.url?scp=33847331083&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.64633-0
DO - 10.1099/ijs.0.64633-0
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C2 - 17267961
AN - SCOPUS:33847331083
SN - 1466-5026
VL - 57
SP - 260
EP - 264
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 2
ER -