TY - JOUR
T1 - Thermodesulfobium acidiphilum sp. nov., a thermoacidophilic, sulfate-reducing, chemoautotrophic bacterium from a thermal site
AU - Frolov, Evgenii N.
AU - Kublanov, Ilya V.
AU - Toshchakov, Stepan V.
AU - Samarov, Nazar I.
AU - Novikov, Andrei A.
AU - Lebedinsky, Alexander V.
AU - Bonch-Osmolovskaya, Elizaveta A.
AU - Chernyh, Nikolai A.
N1 - Publisher Copyright:
© 2017 IUMS.
PY - 2017/5
Y1 - 2017/5
N2 - An obligately anaerobic, sulfate-reducing micro-organism, strain 3127-1T, was isolated from geothermally heated soil (Oil Site, Uzon Caldera, Kamchatka, Russia). The new isolate was a moderately thermoacidophilic anaerobe able to grow with H2 or formate by respiration of sulfate or thiosulfate. The pH range for growth was 3.7–6.5, with an optimum at 4.8–5.0. The temperature range for growth was 37–65 °C, with an optimum at 55 °C. The G+C content of the genomic DNA was 33.7 mol%. The genome of strain 3127-1T contained two almost identical 16S rRNA genes, differing by a single nucleotide substitution. The closest 16S rRNA gene sequence of a validly published species belonged to Thermodesulfobium narugense Na82T (99.5% similarity). However, the average nucleotide identity of the genomes of strain 3127-1T and T. narugense Na82T and the predicted DNA–DNA hybridization value (GGDC 2.1 BLAST+, formula 2) were as low as 86 and 32.5±2.5%, respectively. This, together with phenotypic data, showed the new isolate to belong to a novel species, for which the name Thermodesulfobium acidiphilum sp. nov. is proposed. The type strain is 3127-1T (=DSM 102892T=VKM B-3043T).
AB - An obligately anaerobic, sulfate-reducing micro-organism, strain 3127-1T, was isolated from geothermally heated soil (Oil Site, Uzon Caldera, Kamchatka, Russia). The new isolate was a moderately thermoacidophilic anaerobe able to grow with H2 or formate by respiration of sulfate or thiosulfate. The pH range for growth was 3.7–6.5, with an optimum at 4.8–5.0. The temperature range for growth was 37–65 °C, with an optimum at 55 °C. The G+C content of the genomic DNA was 33.7 mol%. The genome of strain 3127-1T contained two almost identical 16S rRNA genes, differing by a single nucleotide substitution. The closest 16S rRNA gene sequence of a validly published species belonged to Thermodesulfobium narugense Na82T (99.5% similarity). However, the average nucleotide identity of the genomes of strain 3127-1T and T. narugense Na82T and the predicted DNA–DNA hybridization value (GGDC 2.1 BLAST+, formula 2) were as low as 86 and 32.5±2.5%, respectively. This, together with phenotypic data, showed the new isolate to belong to a novel species, for which the name Thermodesulfobium acidiphilum sp. nov. is proposed. The type strain is 3127-1T (=DSM 102892T=VKM B-3043T).
KW - Anaerobe
KW - Geothermally heated soil
KW - Sulfate reductions
KW - Thermoacidophilic bacterium
KW - Thermodesulfobium
UR - http://www.scopus.com/inward/record.url?scp=85020402279&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001745
DO - 10.1099/ijsem.0.001745
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C2 - 27995866
AN - SCOPUS:85020402279
SN - 1466-5026
VL - 67
SP - 1482
EP - 1485
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 5
M1 - 001745
ER -