TY - JOUR
T1 - Thermogemmata fonticola gen. nov., sp. nov., the first thermophilic planctomycete of the order Gemmatales from a Kamchatka hot spring
AU - Elcheninov, Alexander G.
AU - Podosokorskaya, Olga A.
AU - Kovaleva, Olga L.
AU - Novikov, Andrei A.
AU - Toshchakov, Stepan V.
AU - Bonch-Osmolovskaya, Elizaveta A.
AU - Kublanov, Ilya V.
N1 - Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2021/1
Y1 - 2021/1
N2 - A novel aerobic moderately thermophilic bacterium, designated strain 2918T, was isolated from a terrestrial hot spring of Kamchatka, Russian Federation. Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by budding. The strain grew at 25–60 °C and within a pH range of 5.0–8.0 with an optimum at 54–60 °C and pH 7.5. Strain 2918T did not require sodium chloride or yeast extract for growth. It was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (starch, lichenan, galactan, arabinan, xanthan gum, beta-glucan). No growth was observed under anaerobic conditions neither in the presence of sulfur, nitrate, or thiosulfate nor without adding any electron acceptor. Major cellular fatty acids were C18:0 and C20:0. The respiratory quinone was MK-6. The size of the genome of strain 2918T was 4.81 Mb. Genomic DNA G + C content was 60.4 mol%. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 2918T represented a distinct lineage of the order Gemmatales within Planctomycetes. Based on phylogenetic analysis and phenotypic features, the novel isolate was assigned to a novel genus in the Gemmatales for which the name Thermogemmata gen. nov. is proposed. Strain 2918T (=KCTC 72012T =VKM B-3161T) represents its first species Thermogemmata fonticola sp. nov.
AB - A novel aerobic moderately thermophilic bacterium, designated strain 2918T, was isolated from a terrestrial hot spring of Kamchatka, Russian Federation. Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by budding. The strain grew at 25–60 °C and within a pH range of 5.0–8.0 with an optimum at 54–60 °C and pH 7.5. Strain 2918T did not require sodium chloride or yeast extract for growth. It was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (starch, lichenan, galactan, arabinan, xanthan gum, beta-glucan). No growth was observed under anaerobic conditions neither in the presence of sulfur, nitrate, or thiosulfate nor without adding any electron acceptor. Major cellular fatty acids were C18:0 and C20:0. The respiratory quinone was MK-6. The size of the genome of strain 2918T was 4.81 Mb. Genomic DNA G + C content was 60.4 mol%. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 2918T represented a distinct lineage of the order Gemmatales within Planctomycetes. Based on phylogenetic analysis and phenotypic features, the novel isolate was assigned to a novel genus in the Gemmatales for which the name Thermogemmata gen. nov. is proposed. Strain 2918T (=KCTC 72012T =VKM B-3161T) represents its first species Thermogemmata fonticola sp. nov.
KW - Hot spring
KW - Hydrolytic
KW - Kamchatka
KW - Planctomycetes
KW - Thermogemmata
KW - Thermophilic
UR - http://www.scopus.com/inward/record.url?scp=85096618793&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2020.126157
DO - 10.1016/j.syapm.2020.126157
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C2 - 33220635
AN - SCOPUS:85096618793
SN - 0723-2020
VL - 44
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 1
M1 - 126157
ER -