TY - JOUR
T1 - Thermosipho activus sp. nov., a thermophilic, anaerobic, hydrolytic bacterium isolated from a deep-sea sample
AU - Podosokorskaya, Olga A.
AU - Bonch-Osmolovskaya, Elizaveta A.
AU - Godfroy, Anne
AU - Gavrilov, Sergey N.
AU - Beskorovaynaya, Daria A.
AU - Sokolova, Tatyana G.
AU - Kolganova, Tatyana V.
AU - Toshchakov, Stepan V.
AU - Kublanov, Ilya V.
N1 - Publisher Copyright:
© 2014 IUMS.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3T, was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of the novel isolate were rods, 0.3-0.8 µmm in width and 1.5-10 µmm in length, surrounded by a sheath-like structure (toga). Strain Rift-s3T grew at temperatures ranging from 44 to 75 °C, at pH 5.5 to 8.0, and with NaCl concentrations of 3 to 60 g l-1. Under optimum conditions (65 °C, pH 6.0, NaCl 25 g l-1), the doubling time was 30 min. The isolate was able to ferment mono-, oligo-and polysaccharides including cellulose, chitin, xylan and pectin, and proteins including β-keratins, casein and gelatin. Acetate, hydrogen and carbon dioxide were the main products of glucose fermentation. The G+C content of the DNA was 30 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed the affiliation of strain Rift-s3T with the genus Thermosipho, with Thermosipho atlanticus Ob7T as the closest relative (96.5% 16S rRNA gene sequence similarity). Based on the phylogenetic analysis and physiological properties of the novel isolate we propose a novel species of the genus Thermosipho, Thermosipho activus sp. nov., with Rift-s3T (=DSM 26467T=VKM B-2803T) as the type strain.
AB - A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3T, was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of the novel isolate were rods, 0.3-0.8 µmm in width and 1.5-10 µmm in length, surrounded by a sheath-like structure (toga). Strain Rift-s3T grew at temperatures ranging from 44 to 75 °C, at pH 5.5 to 8.0, and with NaCl concentrations of 3 to 60 g l-1. Under optimum conditions (65 °C, pH 6.0, NaCl 25 g l-1), the doubling time was 30 min. The isolate was able to ferment mono-, oligo-and polysaccharides including cellulose, chitin, xylan and pectin, and proteins including β-keratins, casein and gelatin. Acetate, hydrogen and carbon dioxide were the main products of glucose fermentation. The G+C content of the DNA was 30 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed the affiliation of strain Rift-s3T with the genus Thermosipho, with Thermosipho atlanticus Ob7T as the closest relative (96.5% 16S rRNA gene sequence similarity). Based on the phylogenetic analysis and physiological properties of the novel isolate we propose a novel species of the genus Thermosipho, Thermosipho activus sp. nov., with Rift-s3T (=DSM 26467T=VKM B-2803T) as the type strain.
UR - http://www.scopus.com/inward/record.url?scp=84907061563&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.063156-0
DO - 10.1099/ijs.0.063156-0
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C2 - 24994778
AN - SCOPUS:84907061563
SN - 1466-5026
VL - 64
SP - 3307
EP - 3313
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
ER -