TY - JOUR
T1 - Thermotropic behavior of phosphatidylcholine-glucosyl ceramide mixtures
T2 - Effects of phospholipid acyl chain composition and interaction with water
AU - Bach, Diana
AU - Miller, Israel R.
AU - Barenholz, Yechezkel
PY - 1993/7
Y1 - 1993/7
N2 - The thermotropic behavior of multilamellar vesicles composed of mixtures of dimyristoyl phosphatidylcholine-glucosyl ceramide and of egg phosphatidylcholine-glucosyl ceramide was investigated using differential scanning calorimetry. Macroscopic demixing of the lipid components occurred when multilamellar vesicles were prepared from mixtures of glucosyl ceramide and egg phosphatidylcholine by conventional methods. This problem was overcome by a technique based on spray drying of the lipid mixture. The results obtained for the two systems are compared with data available for dipalmitoyl phosphatidylcholine-glucosyl ceramide mixtures (Biochemistry 22 (1983) 3497-3501). All three phosphatidylcholines perturb the complex thermotropic behavior of glucosyl ceramide. The data suggest that the interference with intermolecular interactions among glycosyl ceramide molecules by phospholipid molecules is related to the molecular miscibility of the two components. This is strongly dependent on the acyl chain composition of the phosphatidylcholine and the water activity of the ambient aqueous phase.
AB - The thermotropic behavior of multilamellar vesicles composed of mixtures of dimyristoyl phosphatidylcholine-glucosyl ceramide and of egg phosphatidylcholine-glucosyl ceramide was investigated using differential scanning calorimetry. Macroscopic demixing of the lipid components occurred when multilamellar vesicles were prepared from mixtures of glucosyl ceramide and egg phosphatidylcholine by conventional methods. This problem was overcome by a technique based on spray drying of the lipid mixture. The results obtained for the two systems are compared with data available for dipalmitoyl phosphatidylcholine-glucosyl ceramide mixtures (Biochemistry 22 (1983) 3497-3501). All three phosphatidylcholines perturb the complex thermotropic behavior of glucosyl ceramide. The data suggest that the interference with intermolecular interactions among glycosyl ceramide molecules by phospholipid molecules is related to the molecular miscibility of the two components. This is strongly dependent on the acyl chain composition of the phosphatidylcholine and the water activity of the ambient aqueous phase.
KW - Differential scanning calorimetry
KW - Gaucher's disease
KW - Glucosyl ceramide-phosphatidylcholine
KW - Liposomes
KW - Phase transition and separation
KW - Thermotropic behavior
UR - http://www.scopus.com/inward/record.url?scp=0027320124&partnerID=8YFLogxK
U2 - 10.1016/0301-4622(93)80035-H
DO - 10.1016/0301-4622(93)80035-H
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C2 - 8364149
AN - SCOPUS:0027320124
SN - 0301-4622
VL - 47
SP - 77
EP - 86
JO - Biophysical Chemistry
JF - Biophysical Chemistry
IS - 1
ER -