Abstract
Nucleic acid-functionalized liposomes modified at their boundaries with o-nitrobenzyl phosphate-caged hairpin units and pH-responsive C-G·C+ triplex forming strands are used for the concomitant light and pH-triggered fusion of three types of loaded liposomes. The fusion processes are followed by light-scattering size enlargement measurements, optical methods, and biocatalytic cascades activated upon the mixing of the liposomes loaded with enzymes and their substrates and their fusion into the cell-like containments. The fused liposomes act as functional protocells for the integration of biocatalytic machineries. This is exemplified by the operation of an autonomous polymerization/nickase machinery synthesizing a Mg2+-ion-dependent DNAzyme and of a transcription machinery yielding the Malachite Green-RNA aptamer product.
Original language | English |
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Article number | 2302814 |
Journal | Advanced Functional Materials |
Volume | 33 |
Issue number | 37 |
DOIs | |
State | Published - 12 Sep 2023 |
Bibliographical note
Publisher Copyright:© 2023 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH.
Keywords
- DNAzymes
- photodeprotection
- polymerization/nicking
- transcription
- triplexes