Three Compartment Liposome Fusion: Functional Protocells for Biocatalytic Cascades and Operation of Dynamic DNA Machineries

Fujian Huang*, Huiying Xue, Yuzhe Fu, Yu Ouyang, Danlong Chen, Fan Xia*, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Nucleic acid-functionalized liposomes modified at their boundaries with o-nitrobenzyl phosphate-caged hairpin units and pH-responsive C-G·C+ triplex forming strands are used for the concomitant light and pH-triggered fusion of three types of loaded liposomes. The fusion processes are followed by light-scattering size enlargement measurements, optical methods, and biocatalytic cascades activated upon the mixing of the liposomes loaded with enzymes and their substrates and their fusion into the cell-like containments. The fused liposomes act as functional protocells for the integration of biocatalytic machineries. This is exemplified by the operation of an autonomous polymerization/nickase machinery synthesizing a Mg2+-ion-dependent DNAzyme and of a transcription machinery yielding the Malachite Green-RNA aptamer product.

Original languageEnglish
Article number2302814
JournalAdvanced Functional Materials
Volume33
Issue number37
DOIs
StatePublished - 12 Sep 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH.

Keywords

  • DNAzymes
  • photodeprotection
  • polymerization/nicking
  • transcription
  • triplexes

Fingerprint

Dive into the research topics of 'Three Compartment Liposome Fusion: Functional Protocells for Biocatalytic Cascades and Operation of Dynamic DNA Machineries'. Together they form a unique fingerprint.

Cite this