TY - JOUR
T1 - Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization
AU - Kim, Chong H.
AU - Radermacher, Michael
AU - Kessel, Martin
AU - Frank, Joachim
AU - King, Tsoo E.
PY - 1985
Y1 - 1985
N2 - Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 Å. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.
AB - Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 Å. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.
UR - http://www.scopus.com/inward/record.url?scp=0022031682&partnerID=8YFLogxK
U2 - 10.1016/0162-0134(85)85021-2
DO - 10.1016/0162-0134(85)85021-2
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C2 - 2991453
AN - SCOPUS:0022031682
SN - 0162-0134
VL - 23
SP - 163
EP - 169
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 3-4
ER -