Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

Chong H. Kim; Michael Radermacher; Martin Kessel; Joachim Frank; Tsoo E. King

doi:10.1016/0162-0134(85)85021-2

Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

Chong H. Kim
, Michael Radermacher
, Martin Kessel
, Joachim Frank
, Tsoo E. King^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 Å. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.

Original language	English
Pages (from-to)	163-169
Number of pages	7
Journal	Journal of Inorganic Biochemistry
Volume	23
Issue number	3-4
DOIs	https://doi.org/10.1016/0162-0134(85)85021-2
State	Published - 1985
Externally published	Yes

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title = "Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization",

abstract = "Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 {\AA}. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.",

author = "Kim, \{Chong H.\} and Michael Radermacher and Martin Kessel and Joachim Frank and King, \{Tsoo E.\}",

year = "1985",

doi = "10.1016/0162-0134(85)85021-2",

language = "אנגלית",

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T1 - Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

AU - Kim, Chong H.

AU - Radermacher, Michael

AU - Kessel, Martin

AU - Frank, Joachim

AU - King, Tsoo E.

PY - 1985

Y1 - 1985

N2 - Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 Å. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.

AB - Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0° projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 Å. Our structural results are in agreement with the model of Henderson . [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.

UR - https://www.scopus.com/pages/publications/0022031682

U2 - 10.1016/0162-0134(85)85021-2

DO - 10.1016/0162-0134(85)85021-2

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AN - SCOPUS:0022031682

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VL - 23

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JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 3-4

ER -

Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

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