TY - JOUR
T1 - Three-dimensional Reconstruction of the Surface Protein of the Extremely Thermophilic Archaebacterium Archaeoglobus fulgidus
AU - Kessel, Martin
AU - Volker, Susanne
AU - Santarius, Ute
AU - Huber, Robert
AU - Baumeister, Wolfgang
PY - 1990
Y1 - 1990
N2 - The three-dimensional structure of the regular cell surface of Archaeoglobus fulgidus has been determined to a resolution of 1.8 nm. Envelopes prepared by freezing and thawing the cells were treated for 2 sec with 0.01% SDS to ensure optimal negative staining of the cell surface morphological units, which are arranged on an hexagonal lattice with a center to center spacing of 17.5 nm. The three-dimensional structure reveals the morphological unit to be dome-shaped with a wide opening to the cell membrane and a narrow opening to the exterior at the apex. This design principle is reminiscent of H. volcanii and, to a lesser extent of Sulfolobus solfataricus. The morphological units show little evidence of connectivity and the integrity of the lattice appears to rely on the interaction with the cell membrane.
AB - The three-dimensional structure of the regular cell surface of Archaeoglobus fulgidus has been determined to a resolution of 1.8 nm. Envelopes prepared by freezing and thawing the cells were treated for 2 sec with 0.01% SDS to ensure optimal negative staining of the cell surface morphological units, which are arranged on an hexagonal lattice with a center to center spacing of 17.5 nm. The three-dimensional structure reveals the morphological unit to be dome-shaped with a wide opening to the cell membrane and a narrow opening to the exterior at the apex. This design principle is reminiscent of H. volcanii and, to a lesser extent of Sulfolobus solfataricus. The morphological units show little evidence of connectivity and the integrity of the lattice appears to rely on the interaction with the cell membrane.
KW - Archaebacteria
KW - Archaeoglobus fulgidus
KW - Cell envelope
KW - S-Layer
KW - Three dimensional structure
UR - http://www.scopus.com/inward/record.url?scp=0025061226&partnerID=8YFLogxK
U2 - 10.1016/S0723-2020(11)80187-6
DO - 10.1016/S0723-2020(11)80187-6
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AN - SCOPUS:0025061226
SN - 0723-2020
VL - 13
SP - 207
EP - 213
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 3
ER -